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Title: Physiological and biochemical alterations in Anabaena 7120 under iron stress
Authors: Saxena, R K
Pandey, P K
Bisen, P S
Issue Date: May-2002
Publisher: NISCAIR-CSIR, India
Abstract: Various physiological and biochemical process like growth, NO3--uptake, nitrate reductase, glutamine synthetase and ATPases (Mg2+ and Ca2+ dependent) in the cyanobacterium Anabaena 7120 were observed under iron stress. Growth was found to be maximum in 50 μM  Fe3+ added cells however, 20 μM  Fe3+ (the Fe3+ concentration generally used for routine culturing of cyanobacterial cell in Chu 10 medium) incubation resulted in lower growth. Fe3+ starvation on the other hand showed very poor growth up to 4th day but once the growth started it reached at significant level on 7th day. Higher Fe3+concentration reflected reduced growth with lethality at 500 μM  Fe3+. Chlorophyll a fluorescence under Fe3+ stress reflected almost the similar results as in case of growth. However, the pigment was found to be more sensitive as compared to protein under Fe3+ stress. Similar results have been observed in case of NO3 uptake with only 80% reduction in nutrient uptake in 500 μM  Fe3+ incubated cells. Nitrate reductase activity was lower in Fe3+ starved cells as compared to significant enzyme activity in 20 and 50 μM  Fe3+ incubated cells. Similar to nitrate reductase, glutamine synthetase also showed maximum level in 50 μM Fe3+ added cells, however, higher Fe3+ concentration (300-500 μM ) resulted in reduced enzymatic activity. Glutamine synthetase activity was less sensitivity as compared to nitrate reductase activity under Fe3+ stress. ATPase (Mg2+ and Ca2+ dependent) always showed higher level with increasing Fe3+ concentration.
Page(s): 594-599
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.40(05) [May 2002]

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