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Title: Effect of Alternaria pathotoxin(s) on expression of p53 -like apoptotic protein in calli and leaves of Brassica campestris
Authors: Khandelwal, Abha
Kumar, Anil
Banerjee, Manish
Garg, G K
Issue Date: Jan-2002
Publisher: NISCAIR-CSIR, India
Abstract: Possible involvement of apoptosis was investigated in pathotoxin-treated and nutritionally-depleted in vitro cultured calli by comparing levels of p53-like protein. Antibodies raised against human p53 were used to detect and quantify p53 in B.campestris. Expression of p53-like protein increased from proliferating to static growth stage and reached to constant level at decaying stage. Both ELISA and dot immuno-binding assay showed that p53 -like protein was over expressed in toxin treated and nutritionally depleted calli. Almost similar changes were seen in senescent damage in Brassica species indicating involvement of p53 dependent pathways.
Page(s): 89-94
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.40(01) [January 2002]

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