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Title: Suicidal oxidative stress induced by certain antioxidants
Authors: Koshy, Ligy
Dwarakanath, B S
Raj, H G
Chandra, R
Mathew, T Lazar
Keywords: Antioxidants;Human tumor cell line;Oxidative stress;Pro-oxidant effect;Suicidal oxidative stress
Issue Date: Nov-2003
Publisher: NISCAIR-CSIR, India
Abstract: Well known antioxidants-coumarins (7,8-dihydroxy-4-methyl coumarin -DHMC and 7,8-diacetoxy-4-methyl coumarin DAMC) and flavanoids (quercetin-Q and quercetin penta-acetate-QPA) were investigatcd for their pro-oxidant effects in two human tumor cell lines. The breast carcinoma cell line (MDA-MB-468) was found to be more sensitive to treatment by the drugs- DAMC, Q and QPA at 10μM than the glioma cell line (U-87MG), while DHMC was, non toxic in both cell lines at this concentration. In MDA-MB-468 distinct growth inhibition was observed by 48 hr post treatment. Paradoxically, An increase in the formazan production was revealed by MTT assay at this time indicating an increase in the production of free radicals. An increase in the levels of reactive oxygen species (ROS) was also confirmed by DCFH-DA assay. In cells treated with DAMC, Q and QPA an increase in the percentage of cells with the hypodiploid DNA content was, suggestive of apoptotic cell death. Taken together, these results suggest that an increase in oxidativc stress caused by the pro-oxidant action of these drugs is responsible for cell death.
Page(s): 1273-1278
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.41(11) [November 2003]

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