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Title: High frequency axillary bud multiplication and ex vitro rooting of Wedelia chinensis (Osbeck) Merr. - A medicinal plant
Authors: Martini, K P
Beena, M R
Joseph, Dominic
Issue Date: Mar-2003
Publisher: NISCAIR-CSIR, India
Abstract: An efficient protocol was achieved for rapid propagation of Wedelia chinensis (Osbeck) Merr. through axillary bud proliferation and ex vitro rooting. Murashige and Skoog (MS) medium supplemented with benzyladenine (BA: 8.87 μM) and indole-3 -butyric acid (IBA: 2.46 μM) was optimal for axillary bud proliferation, which developed a mean of 8.3 shoots/node. Excision and cu lture of node segments from in vitro shoots on medium supplemented with the same concentration of growth regulators  developed more than 30 shoots within 40 days. Excision and culture of nodes in success ion enhanced the number of shoots. Shoot multiplication did not exhibit decrease in the number of shoots even at 10th subculture. Nevertheless, the shoots exhibited a tendency towards stunted nature. But reduction of BA to 4.44 or 2.22 μM resumed normal growth of shoots. Half strength MS medium fortified with IBA (2.46 μM) induced the highest number of roots. All in vitro rooted shoots survived in field. Dipping of the basal end of shoots collected from multiplication medium in IBA (2.46μM) solution for 7 days induced roots and its transfer to small pots facilitated the survival of all rooted shoots (100%). Rooting ex vitro by direct transfer of shoots from multiplication medium exhibited 89.2 per cent survival. Use of commercial sugar and tap water and also the commission of in vitro rooting reduce the propagation cost 50-70 per cent. The protocol enables to harvest more than 50,000 plantlets within 150 days starting from a single node explant.
Page(s): 262-266
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.41(03) [March 2003]

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