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|Title:||Production of thiophenes from callus cultures of Tagetes patula L. and its mosquito larvicidal activity|
Ravishanakar, G A
Reddy, B Obul
|Abstract:||Calli were initiated from leaf tissues of T. patula, on Murashige and Skoog's medium supplemented with 2-4 dichlorophenoxacetic acid (2,4-D) and kinetin (Kn). The maximum accumulation of biomass was recorded on 6th week at 1.11 g. Dry wt/culture. Maximum thiophene content was recorded on 4th week at 0.008 % on dry wt basis in callus cultures of T. patula subjected to nutrient stress. Nitrogen stress induced 3-fold increase in thiophene production level in six weeks (0.024 % on dry weight basis). The best hormonal supplementation required for thiophenes production was found to be 2,4-D (2.0 mgL-1) and kinetin (2.0 mg L-1) . The thiophenes produced in callus cultures of T. patula showed larvicidal effect against mosquito larvae.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.41(01) [January 2003]|
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