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Title: Antibacterial and antidiarrhoeal effects of alkaloids of Holarrhena antidysenterica WALL
Authors: Kavitha, D
Shilpa, P N
Devaraj, S Niranjali
Keywords: Holarrhena antiidysenterica;Alkaloids;EPEC;Antidiarrhoeal
Issue Date: Jun-2004
Publisher: NISCAIR-CSIR, India
IPC Code: Int Cl7 A61K
Abstract: The alkaloids from the ethanolic extract of H. antidysenterica seeds were evaluated for their antibacterial activity against clinical isolates of enteropathogenic Escherichia coli (EPEC) in vitro, and their antidiarrhoeal activity on castor oil-induced diarrhoea in rats, in vivo. The pl asmid DNA, whole cell lysate and outer membrane protein profile of a clinical isolate of EPEC was determined in presence of alkaloids of H. antidysenterica. The disc diffusion and agar well diffusion methods were used to evaluate the antibacterial efficacy. The alkaloids showed strong antibacterial activity against EPEC strains. In castor oil –induced diarrhoea, alkaloids reduced the diarrhoea with decrease in the number of wet faeces in pretreated rats at a dose of 200-800 mg/kg. The loss of plasmid DNA and suppression of high molecular weight proteins were observed on alkaloids treatment. Taking into account the multiple antibiotic resistance of EPEC, the results suggest usefulness of alkaloids of H. antidysenterica seeds as antibacterial and antidiarrhoeal agents.
Page(s): 589-594
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.42(06) [June 2004]

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