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Title: Method development and validation of testosterone in muscle by using LC-MS/MS in positive ESI mode
Authors: Dahiya, Meenakshi
Dubey, Nidhi
Singh, Prabha
Singh, G N
Keywords: Testosterone;LC-MS/MS method;Food products;Animal origin;Method development;Validation
Issue Date: Apr-2013
Publisher: NISCAIR-CSIR, India
Abstract: A simple, accurate and precise LC-MS/MS method has been developed and validated for determination of testosterone in muscle. The method has been validated as per the guidelines of ICH and FDA. The finalized method is revealed with significantly by shorter retention time of 2.0 min with simple isocratic programme. The MRM transitions corresponding to m/z 289>97 and 289>109 are used for quantification. The unit of detection of the method is found to be 0.20 ng/kg and unit of quantification has been calculated as 0.40 ng/kg. The method is found to be linear in the range of 0.25 ng/mL to 10 ng/mL. The recoveries of testosterone from the spiked samples of muscles are found between 85-98%. The results of the study show that the proposed method is simple, rapid, precise and accurate, which is useful for the estimation of testosterone in muscle sample and other food products of animal origin with accuracy and reproducibility.
Page(s): 573-578
ISSN: 0975-0983(Online); 0376-4699(Print)
Appears in Collections:IJC-B Vol.52B(04) [April 2013]

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