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Title: 16S rRNA gene sequence analyses of the metagenome derived from waters of river Mahananda at Siliguri: An approach to understand bacterial diversity
Authors: Mukherjee, Shriparna
Kumar, Dhananjay
Nanda, Ashis K
Chakraborty, Ranadhir
Keywords: Amplified ribosomal DNA restriction analysis (ARDRA);Bacterial community;Clone library;Mahananda river;Phylogeny;Ribosomal RNA gene
Issue Date: Jan-2013
Publisher: NISCAIR-CSIR, India
Abstract: Bacterial community of the sub-Himalayan river Mahananda at Siliguri, India was characterized by amplification and sequencing of 16S rRNA genes. Analysis of almost complete 115 sequences revealed a total of 43 operational taxonomic units (OTUs). Statistical analysis revealed that coverage of the clone library was 81.73%. All sequences had >85% similarity to 16S rRNA gene sequences retrieved from the DNA databases. The sequences largely constituted of the class Proteobacteria (10.43% Alpha subdivision, 50.43% Beta subdivision, 9.56% Gamma subdivision & 14.7% Epsilon subdivision), phylum Bacteroidetes (8.69%) and Firmicutes (5.21%), besides single representative belonging to the candidate division OP 11 (0.86%). 31.3% of the total clones showed high similarity to uncultured bacteria, suggesting the usefulness of this technique in uncovering microbial diversity in river water. Abundance of Comamonads (61.76%) among Betaproteobacteria indicated transition to eutrophic state, confirming borderline healthy state of Mahananda River at Siliguri. The most dominant genus of this clone library was Arcobacter, whose presence in environmental waters being associated with fecal pollution has been confirmed.
Page(s): 80-87
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.12(1) [January 2013]

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