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|Title:||Microbial conversion of isoeugenol to vanillin by Rhodococcus rhodochrous|
De, B K
Bhattacharyya, D K
|Abstract:||Microbial biotransformation of isoeugenol to vanillin by the strain Rhodococcuss rhodochrous MTCC 289 has been studied. Optimum conditions for this biotransformation such as temperature, pH, substrate concentration and time course are also established. The strain Rhodochrous MTCC 289 is shake-cultured for 3 days in a medium containing beef extract, yeast extract. peptone and NaCl and 30oC. The bioconversion is carried out with single substrate addition, so that the substrate concentration in the medium do not exceed 1.5% (w/v). The structure of the biotransformation product is established by analytical methods, such as, FTIR, 1H NMR, 13C NMR and GC-MS spectrometry. Under optimal conditions the yield of vanillin in is found to be 58% (molar), demonstrating the efficiency of Rhodococcus rhodochrous for this biotransformation.|
|Appears in Collections:||IJC-B Vol.38B(05) [May 1999]|
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