Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/15996
Title: Isolation and characterization of organic solvent stable protease from alkaliphilic marine Saccharopolyspora species
Authors: Raut, G R
Chakraborty, S
Chopade, B A
Kokare, C R
Keywords: Alkaliphilic;Saccharopolyspora species;Organic solvent stable;Protease;Salt tolerant;Peptide synthesis
Issue Date: Feb-2013
Publisher: NISCAIR-CSIR, India
Abstract: Protease enzyme from thermostable, organic solvent tolerant and less extensively studied marine Sacccharoplyspora species is reported in the present study. Genus of the isolated strain was identified as Saccharopolyspora based on 16S r-DNA sequencing as well as the morphological characteristics and biochemical properties. Optimum pH for cell growth and protease production was pH 10.0. Anionic (SDS) and non-ionic (Tweens) surfactants increased the cell membrane permeability, therefore enhancing the enzyme production. Proteolytic activity of the supernatant of the culture was stable in the presence of various organic solvents. Stability of the enzyme in the presence of organic solvents of the logarithm of the partition coefficient (log P) was equal to or more than 3.2. Enzyme retained 98, 97, 95 and 94% of its activity after the incubation in p-xylene, toluene, n-hexane and n-decane, respectively. Solvent-tolerant microorganism and the solvent-stable enzyme produced by this novel marine Sacccharoplyspora species can be used as biocatalyst for peptide synthesis in the presence of organic solvents.
Page(s): 131-138
URI: http://hdl.handle.net/123456789/15996
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.42(1) [February 2013]

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