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http://nopr.niscair.res.in/handle/123456789/15471| Title: | Immobilization of Amaranthus leaf oxalate oxidase on alkylamine glass |
| Authors: | Pundir, C S Goyal, L Thakur, M Bhargava, A K |
| Issue Date: | Dec-1999 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A membrane bound oxalate oxidase from leaves of Amaranthus - spionsus has been partially purified and immobilized on alkylamine glass with a yield of 9.2 mg protein/g support. The enzyme retained 99.4% of initial activity of free enzyme after immobilization. There was no change in the optimum pH (3 .5) and Vmax but the temperature for maximum activity was slightly decreased (35 °C) and energy of activation (Ea,) and Km for oxalate were increased after immobilization. The immobilized enzyme preparation was stable for 6 months, when stored in distilled water at 4°C. Presence of CI did not affect the activity of immobilized enzyme. |
| Page(s): | 449-452 |
| URI: | http://hdl.handle.net/123456789/15471 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Appears in Collections: | IJBB Vol.36(6) [December 1999] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 36(6) 449-452.pdf | 944.6 kB | Adobe PDF | View/Open |
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