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Title: Immobilization of Amaranthus leaf oxalate oxidase on alkylamine glass
Authors: Pundir, C S
Goyal, L
Thakur, M
Bhargava, A K
Issue Date: Dec-1999
Publisher: NISCAIR-CSIR, India
Abstract: A membrane bound oxalate oxidase from leaves of Amaranthus -  spionsus has been partially purified and immobilized on alkylamine glass with a yield of 9.2 mg protein/g support. The enzyme retained 99.4% of initial activity of free enzyme after immobilization. There was no change in the optimum pH (3 .5) and Vmax but the temperature for maximum activity was slightly decreased (35 °C) and energy of activation (Ea,) and Km for oxalate were increased after immobilization. The immobilized enzyme preparation was stable for 6 months, when stored in distilled water at 4°C. Presence of CI did not affect the activity of immobilized enzyme.
Page(s): 449-452
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.36(6) [December 1999]

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