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|Title:||Immobilization of Amaranthus leaf oxalate oxidase on alkylamine glass|
|Authors:||Pundir, C S|
Bhargava, A K
|Abstract:||A membrane bound oxalate oxidase from leaves of Amaranthus - spionsus has been partially purified and immobilized on alkylamine glass with a yield of 9.2 mg protein/g support. The enzyme retained 99.4% of initial activity of free enzyme after immobilization. There was no change in the optimum pH (3 .5) and Vmax but the temperature for maximum activity was slightly decreased (35 °C) and energy of activation (Ea,) and Km for oxalate were increased after immobilization. The immobilized enzyme preparation was stable for 6 months, when stored in distilled water at 4°C. Presence of CI did not affect the activity of immobilized enzyme.|
|ISSN:||0975-0959 (Online); 0301-1208 (Print)|
|Appears in Collections:||IJBB Vol.36(6) [December 1999]|
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