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Title: Cloning, expression and purification of the DNA binding domain of RFX protein
Authors: Panchal, S C
Madan, Anup
Hosur, R V
Issue Date: Dec-1999
Publisher: NISCAIR-CSIR, India
Abstract: The RFX DNA binding domain (DBD) is a novel highly conserved motif belonging to a large number of dimer DNA binding proteins which have diverse regulatory functions in eukaryotic organisms. To characterize this novel motif, a 78mer polypeptide corresponding to the DBD of human hRFX (hrfX1/DBD), a prototypical member of the RFX family has been cloned and overproduced in Escherichia coli. A purification procedure using cation exchange chromatography has also been developed.
Page(s): 429-432
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.36(6) [December 1999]

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