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Title: n-Propanol as a substrate for assaying the ligninperoxidase activity of Phanerochaete chrysoporium
Authors: Shanmugam, V
Kumari, Meera
Yadav, K D S
Issue Date: Feb-1999
Publisher: NISCAIR-CSIR, India
Abstract: The steady state kinetics of ligninperoxidase catalysed reaction using n-propanol as the organic substrate and monitoring the formation of propanaldehyde at λ = 300 nm spectropbotometerically as functions of different reaction parameters has been studied. It has been concluded that n-propanol can be used as a substrate for analysing the activity of ligninperoxidase. The turnover number of ligninperoxidase of Phanerochaete chrysosporium using n-propanol as substrate has been found to be higher approximately by a factor of 103 as compared to that using veratryl alcohol as the substrate. The method works in assaying the activity of Iigninperoxidase produced by Aspergillus fumigatus indicating that it can be used for assaying the ligninperoxidase activities produced by other microorganisms also and is not limited to assaying the ligninase activity produced by Phanerochaete chrysosporium alone. Under identical experimental conditions, horseradish peroxidase does not show peroxidase activity using n-propanol as substrate indicating that the method does not interfere with the activities of other peroxidases.
Page(s): 39-43
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.36(1) [February 1999]

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