Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/15409
Title: Chemical characterization of the lipopolysaccharides from enteropathogenic Escherichia coli O142 and O158
Authors: Datta, Anup Kr
Basu, Sumanta
Issue Date: Feb-1999
Publisher: NISCAIR-CSIR, India
Abstract: Lipopolysaccharides (LPS) from two enteropathogenic strains of E. coli O142 and O158 were isolated by hot phenol-water extraction procedure. Polyacrylamide gel electrophoretic pattern of the LPS showed the typical ladder like pattern of smooth type of LPS. The LPS of E. coli O158 was  found to contain L-rhamnose, D-glucose and N-acetyl-D-galactosamine as major constituents together with D-galactose, N-acetyl-D-glucosamine, L-glycero-D-manno-heptose and 2-keto-3-deoxy-D-manno-octulosonic acid (KDO) whereas LPS from E. coli O142 contained L-rhamnose, N-acetyl-o-glucosamine and N-acetyl-o-galactosamine as major constituents together with D-glucose, D-galactose, N-acetyl-D-glucosamine, L-glycero-D-mannoheptose and 2-keto-3-deoxy-D-manno-octulosonic acid (KDO). LPS was degraded by mild acid hydrolysis to yield a degraded polysaccharide fraction and an insoluble lipid-A fraction. The main fatty acids of the lipid-A fraction of the LPS were C12:0, C14:0, and 3-OH C14:O for O158 strain whereas E. coli O142 lipid-A consisted of C12:0, C14:0, 3-OH C14:0, and C16:0 . The degraded polysaccharide fraction on gel permeation  chromatography gave a high, moleculer weight O-chain fraction and a core oligosaccharide and a fraction containing degraded sugars. The chemical composition of LPS and its fragmented products are reported in this communication .
Page(s): 55-58
URI: http://hdl.handle.net/123456789/15409
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.36(1) [February 1999]

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