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dc.contributor.authorDinckaya, Erhan-
dc.contributor.authorAkyilmaz, Erol-
dc.contributor.authorAkgol, Sinan-
dc.identifier.issn0975-0959 (Online); 0301-1208 (Print)-
dc.description.abstractA biosensor for the specific determination of uric acid in urine was developed using urate oxidase (EC in combination with a dissolved oxygen probe. Urate oxidase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated teflon membrane to serve as enzyme electrode. The electrode response was maximum when 50 mM glycine buffer was used at pH 9.2 and 35°C. The enzyme electrode response depends linearly on uric acid concentration between 5- 40 μM with a response time of 5 min. The enzyme electrode is stable for more than 2 weeks and during this period over 35 assays were performed.   en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBB Vol.37(1) [February 2000]en_US
dc.titleUrate oxidase electrode based on dissolved oxygen probe for urine uric acid, Determinationen_US
Appears in Collections:IJBB Vol.37(1) [February 2000]

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