Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/15373
Title: Urate oxidase electrode based on dissolved oxygen probe for urine uric acid, Determination
Authors: Dinckaya, Erhan
Akyilmaz, Erol
Akgol, Sinan
Issue Date: Feb-2000
Publisher: NISCAIR-CSIR, India
Abstract: A biosensor for the specific determination of uric acid in urine was developed using urate oxidase (EC 1.7.3.3) in combination with a dissolved oxygen probe. Urate oxidase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated teflon membrane to serve as enzyme electrode. The electrode response was maximum when 50 mM glycine buffer was used at pH 9.2 and 35°C. The enzyme electrode response depends linearly on uric acid concentration between 5- 40 μM with a response time of 5 min. The enzyme electrode is stable for more than 2 weeks and during this period over 35 assays were performed.  
Page(s): 67-70
URI: http://hdl.handle.net/123456789/15373
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.37(1) [February 2000]

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