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|Title:||Urate oxidase electrode based on dissolved oxygen probe for urine uric acid, Determination|
|Abstract:||A biosensor for the specific determination of uric acid in urine was developed using urate oxidase (EC 22.214.171.124) in combination with a dissolved oxygen probe. Urate oxidase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated teflon membrane to serve as enzyme electrode. The electrode response was maximum when 50 mM glycine buffer was used at pH 9.2 and 35°C. The enzyme electrode response depends linearly on uric acid concentration between 5- 40 μM with a response time of 5 min. The enzyme electrode is stable for more than 2 weeks and during this period over 35 assays were performed.|
|ISSN:||0975-0959 (Online); 0301-1208 (Print)|
|Appears in Collections:||IJBB Vol.37(1) [February 2000]|
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