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Title: Cellular fatty acid composition of marine-derived fungi
Authors: Devi, Prabha
Shridhar, M.P. Divya
D’Souza, Lisette
Naik, C.G.
Keywords: Cellular fatty acids;Marine fungi;PUFA;Taxonomy
Issue Date: Dec-2006
Publisher: CSIR
Abstract: The present study quantifies cellular fatty acid composition of four filamentous, marine fungi viz. Trichoderma pseudokoningii, Curvularia lunata, Penicillium chrysogenum and Aspergillus niveus. These fungi were isolated from different marine sources and mass cultured in the laboratory using organic media. Fatty acids were quantified as methyl esters using gas chromatography (GC), using WICHROM software and data collector. This efficient approach using a computer modelled software program provides optimized fatty acid methyl ester (FAME) analyses. The principal fatty acids recorded from these cultures were oleic (18:1 n-9), linoleic (18:2 n-6), palmitic (16:0), stearic (18:0), behenic (22:0), lignoceric (24:0), -linolenic (18:3 n-3), arachidic (20:0), myristic (14:0) and lauric (12:0) in the decreasing order of abundance. Unsaturated fatty acids varied between 57.86% and 85.23% while the saturated fatty acids varied between 14.12% and 41.26% respectively of the total fatty acids. The fatty acids specific to the above mentioned fungi can be used as biomarkers for taxonomic purposes. High concentrations of C18 PUFAs (18:2 n-6 and 18:1 n-9) together with relatively high concentrations of saturated fatty acids like palmitic (16:0) and stearic acids (18:0) accompanied by minor concentrations of lauric (12:0), myristic (14:0), palmitic (16:0), arachidic (20:0), behenic (22:0), lignoceric (24:0), and α-linolenic (18:3 n-3) may be used as reliable biomarkers of the above mentioned cultures. -Linolenic acid, which is an economically important omega-3 polyunsaturated fatty acid was produced less than 1% by all the test cultures. However, T. pseudokoningii (0.58%) and P. chrysogenum (0.68%) may be taken up for manipulation in order to increase the concentration of PUFAs.
Page(s): 359-363
ISSN: 0379-5136
Appears in Collections: IJMS Vol.35(4) [December 2006]

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