NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Geo-Marine Sciences (IJMS) >
IJMS Vol.35 [2006] >
IJMS Vol.35(4) [December 2006] >

Title: Isolation and characterization of four novel thraustochytrid strains from a colonial tunicate
Authors: Rabinowitz, C.
Douek, J.
Weisz, R.
Shabtay, A.
Rinkevich, B.
Keywords: Botryllus
Invertebrate cell culture
Issue Date: Dec-2006
Publisher: CSIR
Abstract: Thraustochytrids, a common group of marine eukaryotic protists, are the major contaminants of invertebrate primary cell cultures. Here we have attempted to isolate thraustochytrid species from primary cultures of extirpated buds of the colonial tunicate Botryllus schlosseri. Four new strains (termed BS3-BS6) were isolated. Cells of these strains subjected to three media conditions, showed different growth patterns and cellular morphologies. Phylogenetic (18S rDNA) and AFLP analyses revealed two-band patterns, one characteristic to strains BS3 and BS5 and the other to strains BS4 and BS6. A Sequence analysis showed 98 % identity between strains BS3 and BS5, and 100 % identity between strains BS4 and BS6. Distance analysis tree showed that the four new strains were primarily related to two other thraustochytrids strains, independently isolated from the same tunicate species a few years ago. It is suggested that the six closely related thraustochytrid strains isolated from Botryllus schlosseri reveal a biological association between the tunicate and a clade of species/cryptic species of thraustochytrids, which can be identified by molecular markers, growth patterns and cellular characterizations.
Page(s): 341-350
ISSN: 0379-5136
Source: IJMS Vol.35(4) [December 2006]

Files in This Item:

File Description SizeFormat
IJMS 35(4) 341-350.pdf537.12 kBAdobe PDFView/Open
 Current Page Visits: 108 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 167632 since 01-Sep-2015  Last updated on 28-Jun-2016Webmaster: