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dc.contributor.authorSingh, Rakesh K-
dc.contributor.authorVerma, Naresh C-
dc.contributor.authorKagiya, V T-
dc.identifier.issn0975-0959 (Online); 0301-1208 (Print)-
dc.description.abstractThe radioprotection conferred by a highly water soluble glucose derivative of α -tocopherol, namely, 2-( α-D-glucopyranosyl) methyl-2.5,7,8-tetramethylchroman-6-ol (TMG) in Saccharomyces cerevisiae was studied. Cells grown in standard YEPD-agar medium and irradiated in the presence of TMG showed a concentration dependent higher survival up to 10 mM of TMG in comparison to cells irradiated in distilled water. Treatment of TMG to cells given either before or immediately after irradiation but not during irradiation, had no effect on their radiation response. S.cerevisiae strain LP1383 (rad52) which is defective in recombination repair showed enhanced radioresistance only when subjected to irradiation in presence of TMG. Cells of radS2 strain grown in the medium containing TMG showed a radiation response similar to that of cells grown in the medium without TMG. The nature of TMG dependent enhanced radioresistance was studied by scoring the mutations in the strain D-7, which behaved like wild type strain in complete medium, at trp and ilv loci . Our study indicated that TMG confers radioresistance in S. cerevisiae possibly by two mechanisms viz. (i), by eliminating radiation induced reactive free radicals when the irradiation is carried out in the presence of TMG and (ii ), by activating an error - prone repair process involving RAD52 gene, when the cells are grown in the medium containing TMG.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBB Vol.38(6) [December 2001]en_US
dc.titleEffect of a water soluble derivative of α-tocopherol on radiation response of Saccharomyces cerevisiaeen_US
Appears in Collections:IJBB Vol.38(6) [December 2001]

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