Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/15306
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKurien, B T-
dc.contributor.authorMatsumoto, H-
dc.contributor.authorScofield, R H-
dc.date.accessioned2012-12-25T18:26:41Z-
dc.date.available2012-12-25T18:26:41Z-
dc.date.issued2001-08-
dc.identifier.issn0975-0959 (Online); 0301-1208 (Print)-
dc.identifier.urihttp://hdl.handle.net/123456789/15306-
dc.description274-276en_US
dc.description.abstractA simple procedure for the purification of tryptic peptides, prior to mass spectrometric analysis, using polyvinylidene fluoride membrane (PVDF) is described. The sensitivity of mass spectrometric analysis is such that minor impurities in tryptic peptide digests suppress the signal obtained. However, we obtained useful signal, from a sample that did not yield any spectra earlier, by purifying the sample using PVDF membrane. For this. the tryptic peptide digest was first spotted on the membrane which was then air-dried and washed. Further, the membrane was extracted with trifluoroacetic acid (TFA) and acetonitrile and subjected to mass spectrometric analysis. This procedure enabled us to identify a cross-reactive D1 antigen on the neutrophil surface that bound antibodies that targeted 60 kD Ro autoantigen in systemic lupus erythematosus, an autoimmune disorder.en_US
dc.language.isoen_USen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBB Vol.38(4) [August 2001]en_US
dc.titlePurification of tryptic peptides for mass spectrometry using polyvinylidene fluoride Membraneen_US
dc.typeArticleen_US
Appears in Collections:IJBB Vol.38(4) [August 2001]

Files in This Item:
File Description SizeFormat 
IJBB 38(4) 274-276.pdf723.93 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.