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|Title:||Resolution of a complex ionic mixture of an apparently homogenous protein preparation by preparative electrophoresis|
|Authors:||Dubey, Ashok K|
|Abstract:||Preparations of recombinant envelope glycoprotein E2 of hepatitis C virus (r-HCV E2), found to be homogeneous by N-terminal amino acid sequencing and mass spectrometry, resolved into multiple ionic species (isoforms) when analysed by isoelectric focusing (IEF) gel electrophoresis in the pI range of 3-10. These isoforms possessed pI values in the range or 4.5-8.2. The major isoform with pI value of approximately 7. 1 was separated from the rest of them by employing a method developed on Gradiflow BF 200, a device based on preparative electrophoresis. This isoform was adjudged to be homogenous by IEF and by native polyacrylamide gel electrophoresis (PAGE).|
|ISSN:||0975-0959 (Online); 0301-1208 (Print)|
|Appears in Collections:||IJBB Vol.39(4) [August 2002]|
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