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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(6) [December 2012] >
| Title: | Nanoactivator mediated modifications in thermostable amylase from Bacillus licheniformis |
| Authors: | Khairnar, Rajendra S
Mahabole, Megha P
Pathak, Anupama P |
| Keywords: | Bacillus
Extremophiles
Nanoactivator
Nanomaterials
Thermophiles
Thermostable amylase |
| Issue Date: | Dec-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Gram-positive rod-shaped thermophilic bacteria were isolated using
samples collected from terrestrial natural thermal spring located at Unkeshwar (Longitude 78.22 degree East to 78.34 degree
East, Latitude 19 degree 34' North to 19 degree 40' North), District
Nanded, Maharashtra State, India. The isolates were
then cultivated using selective media and identified using
culture-dependent techniques. One prominent isolate (UN1) exhibited high
temperature stability and remarkable amylase production and was identified as Bacillus licheniformis. Amylase
production was carried out in starch media and the enzyme was partially
purified and characterized for optimization of pH and temperature. Amylolytic
activity of the enzyme was determined. Nanoactivator-mediated modifications
were carried out to enhance amylolytic activity of the partially purified
amylase. Three-fold increase in catalytic efficiency of amylase was obtained
after modification.
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| Page(s): | 468-471 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(6) [December 2012]
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