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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(6) [December 2012] >
| Title: | Probing the evolutionary conserved regions within functional site of drug-resistant target proteins of Staphylococcus aureus: In silico phylogenetic motif profiling approach |
| Authors: | Kahlon, Amandeep Kaur
Darokar, Mahendra P
Sharma, Ashok |
| Keywords: | Multi-drug resistance
Staphylococcus aureus
Domain
Co-target
Phylogenetic similarity threshold
Phylogenetic motifs
Interactome analysis
Drug-resistant genes
Conserved residues
Functional partners |
| Issue Date: | Dec-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Staphylococcus
aureus is one of the major causes of clinical
infections and increasing mortality due to multi-drug resistance. In this
study, eight drug-resistant genes, beta-lactamase, metallo-beta-lactamase,
vanB, mecA, norA, qacA, qacB and qacC of S.
aureus strain Mu50 (vancomycin resistant) were studied to predict the
evolutionary conserved functional site residues in their protein sequences. It
was found that in beta-lactamase, Tyr, Gly, Thr, Asn and in
metallo-beta-lactamase, Thr, His, Gly, Leu, Arg and Asp residues were highly
conserved. In vanB, Gly, His and Asp residues were highly conserved. Whereas in
mecA, His, Val, Phe, Gln, Lys and in norA, Ser, Leu and Ala residues showed conservedness at
moderate level. In the multi-drug efflux pump (corresponding to qacA, qacB and
qacC), Gly residue was found to be highly conserved. The homology clustering of
target proteins through SCI-PHY algorithm and homologues identified through
PSI-BLAST were compared to identify the degree of conservation of functional
residues. The phylogenetic motifs identified using homologues of target
proteins were validated through domain search to locate their site and
functionality in the protein sequences. Interactome analysis was performed to
understand the possible mode of interaction of target proteins with their
functional partners. |
| Page(s): | 442-450 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(6) [December 2012]
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