Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/15239
Title: <i style="mso-bidi-font-style: normal">GbWRKY1</i>, a novel cotton (<i style="mso-bidi-font-style: normal">Gossypium barbadense</i>) WRKY gene isolated from a bacteriophage full-length cDNA library, is induced by infection with <i style="mso-bidi-font-style:normal">Verticillium dahliae</i>
Authors: Shu-Ling, Zhang
Xing-Fen, Wang
Yan, Zhang
Jian-Feng, Liu
Li-Zhu, Wu
Dong-Mei, Zhang
Zhi-Ying, Ma
Keywords: cDNA-Expression library
<i style="mso-bidi-font-style:normal"><span style="font-size:9.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">Gossypium barbadense</span></i>
<i style="mso-bidi-font-style:normal"><span style="font-size:9.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">Verticillium</span></i><span style="font-size:9.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB"> wilt resistance</span>
<i style="mso-bidi-font-style:normal"><span style="font-size:9.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">WRKY</span></i><span style="font-size:9.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB"> gene</span>
Issue Date: Dec-2012
Publisher: NISCAIR-CSIR, India
Abstract: WRKY transcription factor proteins play important roles in diverse stress responses. In this study, we first cloned a novel <i style="mso-bidi-font-style:normal">WRKY </i>from our constructed bacteriophage full-length cDNA library for cotton (<i style="mso-bidi-font-style:normal">Gossypium barbadense</i>). The plants were stressed by exposure to a defoliating strain of <i style="mso-bidi-font-style:normal">Verticillium dahliae</i>. The capacity of primary cDNA library was 1.28 × 10<sup>6</sup> PFU and the titer of the amplified cDNA library was >10<sup>10 </sup>PFU mL<sup>–1</sup>. The recombination rate of the library was 94% and average insert size was about 1.1 kb. This novel gene, named <i style="mso-bidi-font-style:normal">GbWRKY1</i> was 1971 bp long and encodes a protein of 489 amino acids. It contains two characteristic WRKY domains and two zinc finger motifs. The sub-cellular assay indicated that <i style="mso-bidi-font-style:normal">Gb</i>WRKY1–GFP fusion protein was localized in the nucleus. Furthermore, Northern blot analysis showed that expression pattern of <i style="mso-bidi-font-style:normal">GbWRKY1 </i>was similar among tissue types (roots, stems and leaves), but differed between pathogen-infiltrated and Czapek medium-infiltrated (untreated control) plants. Quantitative real-time PCR showed that <i style="mso-bidi-font-style: normal">GbWRKY1</i> could also be induced by salicylic acid (SA), methyl jasmonate (MeJA) and 1-aminocyclopropane-1-carboxylic acid (ACC). <span style="mso-ansi-language:EN" lang="EN">These findings clearly suggest that as a pathogen-inducible transcription factor <i style="mso-bidi-font-style:normal">GbWRKY1</i> plays an important role in plant defense responses. </span>
Description: 405-413
URI: http://hdl.handle.net/123456789/15239
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.49(6) [December 2012]

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