Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/15237
Title: Effects of low intensity electromagnetic irradiation of 70.6 and 73 GHz frequencies and antibiotics on energy-dependent proton and potassium ion transport by<i> E. coli</i>
Authors: Torgomyan, H
Keywords: Extremely high frequency electromagnetic irradiation
<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";letter-spacing:-.3pt;mso-ansi-language: EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">E. coli</span></i>
Ion transport
Antibiotics
<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";letter-spacing:-.3pt;mso-ansi-language: EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">N,N’</span></i><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";letter-spacing:-.3pt;mso-ansi-language: EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">-dicyclohexycarbodiimide</span>
Issue Date: Dec-2012
Publisher: NISCAIR-CSIR, India
Abstract: The effects of low intensity (flux capacity 0.06 mW/cm<sup>2</sup>) coherent electromagnetic irradiation (EMI) of 70.6 and 73 GHz frequencies and their combined effects with antibiotics — ceftriaxone or kanamycin (0.4 or 15 µM, correspondingly) on <i style="mso-bidi-font-style:normal">E. coli</i> K12 growth and survival have been reported previously. To further study the effects of EMI and antibiotics and mechanisms, decrease in overall energy (glucose)-dependent H<sup>+</sup> and K<sup>+</sup> fluxes across the cell membrane was investigated in <i style="mso-bidi-font-style:normal">E. coli</i>. The depression of H<sup>+</sup> and K<sup>+</sup> fluxes rate was maximally achieved with the 73 GHz frequency. The EMI strengthened the effect of <i style="mso-bidi-font-style:normal">N,N’</i>-dicyclohexycarbodiimide (DCCD, an inhibitor of the F<sub>0</sub>F<sub>1</sub>-ATPase). The 73 GHz EMI had more influence on H<sup>+</sup> efflux inhibition, whereas 70.6 GHz on K<sup>+</sup> influx. Also, EMI strengthened the depressive effects of ceftriaxone and kanamycin on the overall and DCCD-inhibited H<sup>+</sup> and K<sup>+</sup> fluxes. The 73 GHz EMI strengthened the effect of ceftriaxone on both ions fluxes. Kanamycin depressed H<sup>+</sup> efflux more as compared to ceftriaxone, which was also strengthened with EMI. The results of <i style="mso-bidi-font-style:normal">E. coli</i> H<sup>+</sup> and K<sup>+</sup> transport systems activities depression by irradiation and the irradiation effect on DCCD and antibiotics action indicated the EMI and antibiotics causing primary changes in the bacterial membrane.
Description: 428-434
URI: http://hdl.handle.net/123456789/15237
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.49(6) [December 2012]

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