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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(6) [December 2012] >
| Title: | Effects of low intensity electromagnetic irradiation of 70.6 and 73 GHz frequencies and antibiotics on energy-dependent proton and potassium ion transport by E. coli |
| Authors: | Torgomyan, H |
| Keywords: | Extremely high frequency electromagnetic irradiation
E. coli
Ion transport
Antibiotics
N,N’-dicyclohexycarbodiimide |
| Issue Date: | Dec-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The effects of low intensity (flux capacity 0.06 mW/cm2)
coherent electromagnetic irradiation (EMI) of 70.6 and 73 GHz frequencies and
their combined effects with antibiotics — ceftriaxone or kanamycin (0.4 or 15 µM,
correspondingly) on E. coli K12
growth and survival have been reported previously. To further study the effects
of EMI and antibiotics and mechanisms, decrease in overall energy
(glucose)-dependent H+ and K+ fluxes across the cell
membrane was investigated in E. coli.
The depression of H+ and K+ fluxes rate was maximally
achieved with the 73 GHz frequency. The EMI strengthened
the effect of N,N’-dicyclohexycarbodiimide
(DCCD, an inhibitor of the F0F1-ATPase). The 73 GHz EMI
had more influence on H+ efflux inhibition, whereas 70.6 GHz on K+
influx. Also, EMI strengthened the
depressive effects of ceftriaxone and kanamycin on the overall and
DCCD-inhibited H+ and K+ fluxes. The 73 GHz EMI strengthened the effect of ceftriaxone on both
ions fluxes. Kanamycin depressed H+ efflux more as compared to
ceftriaxone, which was also strengthened with
EMI. The results of E. coli H+
and K+ transport systems activities depression by irradiation and
the irradiation effect on DCCD and antibiotics action indicated the EMI and
antibiotics causing primary changes in the bacterial membrane. |
| Page(s): | 428-434 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(6) [December 2012]
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