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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(6) [December 2012] >
| Title: | Anti-proliferative effect of leaf extracts of Eucalyptus citriodora against human cancer cells in vitro and in vivo |
| Authors: | Bhagat, Madhulika
Sharma, Vikas
Saxena, Ajit Kumar |
| Keywords: | Eucalyptus citriodora
Cytotoxicity
Human cancer cell lines
Murine tumor model
Ethyl acetate extract |
| Issue Date: | Dec-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Six different extracts from
Eucalyptus citriodora leaves were investigated for their anticancer effect.
Extracts were prepared using a range of polar and non-polar solvents to leach
out maximum active components. Phytochemical analysis of the extracts revealed
the presence of anthraquinones, cardiac glycosides, flavonoids, saponins and
tannins. Cytotoxic activity of
different extracts was tested in vitro
against seven human cancer cell lines from seven different tissues, such as SW-620
(colon), HOP-62 (lung), PC-3 (prostate), OVCAR-5 (ovary), HeLa (cervix), IMR-32
(neuroblastoma) and HEP-2 (liver). The ethyl acetate, chloroform and 50%
methanolic extract displayed highest anti-proliferative effect in a dose-dependent
manner. In vivo anti-tumor activity
was evaluated against murine tumor (solid) model of Ehrlich ascites carcinoma and
Sarcoma 180. The
results showed that ethyl acetate and aqueous extracts suppressed the growth of Ehrlich ascites carcinoma (29.79% and 18.48%, respectively),
but showed little growth inhibition in case of Sarcoma 180 (13. 86% and 8.57%, respectively). The activity might be due to the flavonoids, tannins and
saponins that are present in all the extracts of the plant. Further
investigation is required for the isolation of active principle(s) from the
ethyl acetate extract, which has shown significant in vitro and in vivo
anticancer potential. |
| Page(s): | 451-457 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(6) [December 2012]
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