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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.39 [2002] >
IJBB Vol.39(5) [October 2002] >
| Title: | Purification and properties of invertase from the flowers of Woodfordia fruticosa |
| Authors: | Weersaooriya, M K B
Yatawara, H P |
| Issue Date: | Oct-2002 |
| Publisher: | CSIR |
| Abstract: | Invertase (β- fructofuranosidase, EC 3.2.1.26) was purified
from the flowers of of
Woodfordia fruticosa, which is used to prepare
certain fermented Ayurvedic drugs.
The enzyme was purified to near homogeneity as judged by native PAGE with a
yield of 10.7%, using
(N H4)2SO4
fractionation, followed by gelfiltration through Sepharose 4B and DEAE
cellulose chromatography at pH 6.8
and 4.42. The molecular mass of the purified enzyme as determined by elution
through Sepharose 4B gel column was found to be ~ 280 kDa. SDS-PAGE of the
purified enzyme showed that the enzyme is composed of three subunits with
molecular mass of 66. 43 and 40 kDa. The
enzyme showed a broad pH optimum between
4.0-7.0. Optimum assay temperature was 37°C and above 45°C, the enzyme activity
slowly declined and inactivated around 80°C. The apparent Km value
of th e enzyme for sucrose was 160mM. |
| Page(s): | 347-350 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.39(5) [October 2002]
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