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Title: Purification and properties of invertase from the flowers of <i>Woodfordia fruticosa</i>
Authors: Weersaooriya, M K B
Yatawara, H P
Issue Date: Oct-2002
Publisher: CSIR
Abstract: Invertase <span style="font-size:14.0pt;font-family:HiddenHorzOCR;mso-hansi-font-family:Arial; mso-bidi-font-family:HiddenHorzOCR">(β<span style="font-size:14.0pt;font-family:HiddenHorzOCR;mso-hansi-font-family:Arial; mso-bidi-font-family:HiddenHorzOCR">- fructofuranosidase, EC was purified from the  flowers of of  <i>Woodfordia fruticosa</i><i>,  </i>which is used to prepare certain fermented Ayurvedic drugs. The enzyme was purified to near homogeneity as judged by native PAGE with a yield of 10.7%, using (N H<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> fractionation, followed by gelfiltration through Sepharose 4B and DEAE cellulose chromatography at <i style="mso-bidi-font-style:normal">p</i>H 6.8 and 4.42. The molecular mass of the purified enzyme as determined by elution through Sepharose 4B gel column was found to be ~ 280 kDa. SDS-PAGE of the purified enzyme showed that the enzyme is composed of three subunits with molecular mass of  66. 43 and 40 kDa. The enzyme showed a broad <i style="mso-bidi-font-style:normal">p</i>H optimum between 4.0-7.0. Optimum assay temperature was 37°C and above 45°C, the enzyme activity slowly declined and inactivated around 80°C. The apparent K<sub>m</sub> value of th e enzyme for sucrose was 160m<i style="mso-bidi-font-style:normal">M</i>.<span style="font-size:14.0pt;font-family:HiddenHorzOCR;mso-hansi-font-family:Arial; mso-bidi-font-family:HiddenHorzOCR"><span style="font-size:14.0pt;font-family:HiddenHorzOCR;mso-hansi-font-family:Arial; mso-bidi-font-family:HiddenHorzOCR"><span style="font-size:14.0pt;font-family:Arial;mso-fareast-font-family:" times="" new="" roman";="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"=""></span></span></span></span></span>
Description: 347-350
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.39(5) [October 2002]

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