Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/15179
Title: <span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-US">Direct and indirect method of plant regeneration from root explants of <i style="mso-bidi-font-style:normal">Caesalpinia bonduc</i> (L.) Roxb.— A threatened medicinal plant of Western Ghats</span>
Authors: Kumar, S R Santosh
Krishna, V
Venkatesh
Pradeepa, K
Kumar, K Girish
Gnanesh, A U
Keywords: <i><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language: EN-US;mso-bidi-language:AR-SA" lang="EN-US">Caesalpinia bonduc</span></i>
Plant regeneration
Root explants
Issue Date: Dec-2012
Publisher: NISCAIR-CSIR, India
Abstract: An <i style="mso-bidi-font-style:normal">in vitro</i> regeneration protocol has been standardized via direct and indirect methods from excised root explants of<i> C. bonduc,</i> a threatened woody legume used for the treatment of contagious diseases, inflammation, leprosy, antiperiodic, febrifuge, anthelmenthic, urinary disorders, leucorrhoea, piles and to heal wounds. MS medium supplemented with 17.75 µmol BAP and 2.46 µmol IBA, induced a mean of 3.40 ± 1.07 shoots directly from the surface of excised root explant. Subsequently, the shoots rooted readily on MS half strength medium with out growth regulators. In indirect organogenesis, callogenic frequency was optimized (96.66%) at the concentration of 9.04 µmol 2, 4-D and 0.88 µmol BAP. An average, 15.30 ± 5.25 shoots were differentiated from the root callus at the concentration of 17.57 µmol BAP and 2.85 µmol IAA. Shoots regenerated through callus were rooted well on MS half strength medium with growth regulators at 2.95 µmol IBA. Rooted plantlets were transferred to the pots containing sterilized soil and were successfully hardened at greenhouse condition for three weeks then exposed to the natural environment. Survival rate was more (95%) in plantlets derived through direct organogenesis than (60%) the plantlets regenerated through root calli.
Description: 910-917
URI: http://hdl.handle.net/123456789/15179
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.50(12) [December 2012]

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