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IJEB Vol.50 [2012] >
IJEB Vol.50(12) [December 2012] >
| Title: | Direct and indirect method of plant regeneration from root explants of Caesalpinia bonduc (L.) Roxb.— A threatened medicinal plant of Western Ghats |
| Authors: | Kumar, S R Santosh
Krishna, V
Venkatesh
Pradeepa, K
Kumar, K Girish
Gnanesh, A U |
| Keywords: | Caesalpinia bonduc
Plant regeneration
Root explants |
| Issue Date: | Dec-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An in vitro
regeneration protocol has been standardized via direct and indirect methods
from excised root explants of
C. bonduc, a threatened woody legume used for the treatment of contagious
diseases, inflammation, leprosy, antiperiodic, febrifuge, anthelmenthic,
urinary disorders, leucorrhoea, piles and to heal wounds. MS medium
supplemented with
17.75 µmol BAP and 2.46 µmol IBA, induced a mean of 3.40 ± 1.07 shoots directly
from the surface of excised root explant. Subsequently, the shoots rooted
readily on MS half strength medium with out growth regulators. In indirect
organogenesis, callogenic frequency was optimized (96.66%) at the concentration
of 9.04 µmol 2, 4-D and 0.88 µmol BAP. An average, 15.30 ± 5.25 shoots were
differentiated from the root callus at the concentration of 17.57 µmol BAP and
2.85 µmol IAA. Shoots regenerated through callus were rooted well on MS half
strength medium with growth regulators at 2.95 µmol IBA. Rooted plantlets were
transferred to the pots containing sterilized soil and were successfully
hardened at greenhouse condition for three weeks then exposed to the natural
environment. Survival rate was more (95%) in plantlets derived through direct
organogenesis than (60%) the plantlets regenerated through root calli. |
| Page(s): | 910-917 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(12) [December 2012]
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