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Title: Induction of somatic embryogenesis in endangered butterfly ginger Hedychium coronarium J. Koenig
Authors: Verma, Manju
Bansal, Y K
Keywords: Callus culture
Hedychium coronarium
Medicinal plant
Somatic embryogenesis
Issue Date: Dec-2012
Publisher: NISCAIR-CSIR, India
Abstract: An efficient protocol has been developed for regeneration of complete plants through somatic embryogenesis in H. coronarium. Creamish white, pale yellow and brown calli were obtained on MS medium supplemented with different concentrations of auxins [2, 4-Dichlorophenoxy acetic acid (2, 4-D), Indole-3 acetic acid (IAA) and 1-Naphthylacetic acid (NAA)] after 4 weeks. Creamy white calli developed on 0.5 mg L-1 2, 4-D turned embryogenic when subcultured on basal medium and produced small globular somatic embryos after 6 weeks. Further growth of somatic embryos required their transfer to medium containing 6-benzylaminopurine (BAP) or kinetin (KN). BAP was more effective than KN in promoting shoot proliferation. Maximum shoot length was obtained with 0.5 mg L-1 BAP whereas maximum shoot number was obtained with 1.0 mg L-1 BAP. The plantlets thus formed were successfully hardened, and transferred to sand-soil and farm yard manure (1:1:1) with 95% survival.
Description: 904-909
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.50(12) [December 2012]

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