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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(12) [December 2012] >
| Title: | Induction of somatic embryogenesis in endangered butterfly ginger Hedychium coronarium J. Koenig |
| Authors: | Verma, Manju
Bansal, Y K |
| Keywords: | Callus culture
Hedychium coronarium
Medicinal plant
Somatic embryogenesis |
| Issue Date: | Dec-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An efficient protocol has been developed for regeneration
of complete plants through somatic embryogenesis in
H. coronarium. Creamish white, pale yellow and brown calli
were obtained on MS
medium supplemented with different concentrations of auxins [2, 4-Dichlorophenoxy acetic acid (2, 4-D), Indole-3 acetic acid (IAA) and 1-Naphthylacetic acid (NAA)] after 4 weeks. Creamy white calli developed
on 0.5 mg L-1 2, 4-D turned embryogenic when subcultured on basal medium
and produced
small globular somatic embryos after 6 weeks. Further growth of somatic embryos
required their transfer to medium containing 6-benzylaminopurine (BAP) or kinetin (KN). BAP was more effective than KN in promoting shoot proliferation. Maximum shoot length was obtained with 0.5 mg L-1 BAP whereas maximum
shoot number was obtained with 1.0 mg L-1 BAP. The plantlets thus formed were successfully hardened,
and transferred to sand-soil and farm yard manure (1:1:1) with 95% survival. |
| Page(s): | 904-909 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(12) [December 2012]
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