Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/15178
Title: <span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">Induction of <span style="font-size:11.0pt;mso-bidi-font-size:10.0pt; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">somatic embryogenesis in endangered butterfly ginger <i>Hedychium coronarium </i>J. Koenig</span></span>
Authors: Verma, Manju
Bansal, Y K
Keywords: Callus culture
<i><span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Hedychium coronarium</span></i>
Medicinal plant
Somatic embryogenesis
Issue Date: Dec-2012
Publisher: NISCAIR-CSIR, India
Abstract: <span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:ar-sa;="" mso-bidi-font-weight:bold"="" lang="EN-GB">An<span style="font-size:11.0pt; mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-gb;mso-fareast-language:en-us;="" mso-bidi-language:hi"="" lang="EN-GB"> efficient protocol has been developed for regeneration of complete plants through somatic embryogenesis in <i style="mso-bidi-font-style:normal">H. coronarium</i>.<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-gb;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="" lang="EN-GB"> Creamish white, pale yellow and brown calli were obtained <span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB">on MS medium supplemented with different concentrations of auxins [<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-gb;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="" lang="EN-GB">2, 4-Dichlorophenoxy acetic acid <span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-gb;mso-fareast-language:="" en-us;mso-bidi-language:hi"="" lang="EN-GB">(2, 4-D), <span style="font-size: 11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-gb;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-GB">Indole-3 acetic acid (<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-gb;mso-fareast-language:="" en-us;mso-bidi-language:hi"="" lang="EN-GB">IAA) and <span style="font-size: 11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-gb;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-GB">1-Naphthylacetic acid (<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-gb;mso-fareast-language:="" en-us;mso-bidi-language:hi"="" lang="EN-GB">NAA)] after 4 weeks. Creamy white calli developed on <span style="font-size:11.0pt;mso-bidi-font-size:10.0pt; font-family:" times="" new="" roman";mso-fareast-font-family:hnewsserif;mso-ansi-language:="" en-gb;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB">0.5 mg L<sup>-1</sup> <span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-gb;mso-fareast-language:="" en-us;mso-bidi-language:hi"="" lang="EN-GB">2, 4-D <span style="font-size: 11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-gb;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-GB">turned embryogenic when subcultured on basal medium and <span style="font-size:11.0pt;mso-bidi-font-size:10.0pt; font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB">produced small globular somatic embryos<span style="font-size:11.0pt; mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-gb;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-GB"> after 6 weeks. Further growth of somatic embryos required their transfer to medium containing <span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:hnewsserif;mso-ansi-language:en-gb;mso-fareast-language:="" en-us;mso-bidi-language:hi"="" lang="EN-GB">6-benzylaminopurine (BAP) or kinetin (KN).<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-gb;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="" lang="EN-GB"> <span style="font-size:11.0pt; mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" hnewsserif;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">BAP was more effective than KN in promoting shoot proliferation. M<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-gb;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="" lang="EN-GB">aximum <span style="font-size: 11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" hnewsserif;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">shoot length was obtained with 0.5 mg L<sup>-1</sup> BAP whereas maximum shoot number was obtained with 1.0 mg L<sup>-1</sup> BAP.<b><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-gb;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="" lang="EN-GB"> </span></b><span style="font-size: 11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-gb;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-GB">The plantlets thus formed were successfully hardened, and transferred to sand-soil and farm yard manure (1:1:1) with 95% survival.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
Description: 904-909
URI: http://hdl.handle.net/123456789/15178
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.50(12) [December 2012]

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