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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(12) [December 2012] >
| Title: | Effect of long acting insulin supplementation on diabetic nephropathy in Wistar rats |
| Authors: | Michael, Shiju Thomas
Ganesh, Rajesh Nachiappa
Viswanathan, Pragasam |
| Keywords: | Diabetic nephropathy
Glargine
Long acting insulin
Rat model
Streptozotocin |
| Issue Date: | Dec-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This
study was designed to check whether insulin supplementation is crucial for
inducing diabetic nephropathy (DNP) in Wistar rats. Diabetes was induced by a
single intraperitoneal injection of streptozotocin. The urinary biochemical
parameters such as albumin, creatinine and urea nitrogen were monitored every
two weeks. The histological changes in the kidney were observed at the end of
both fifth and seventh month. Immunohistochemical analyses of VEGF, ERK-1 and
NF- B expression were performed to demonstrate mesangial expansion and
glomerulosclerosis, which are the defining histological features of
nephropathy. A significant change in the urinary biochemistry was observed in
diabetic animals at the end of four months, but the aforementioned quantitative
changes were delayed in diabetic animals treated with insulin. At the end of
seven months, the diabetic animals showed prominent histological changes such
as glomerular basement membrane thickening, nodular glomerulosclerosis and
mesangial expansion. However, these changes were not observed in diabetic
animals treated with insulin even at the end of the study. From the results, it
can be concluded that there is no need of insulin supplementation for inducing
DNP, when the animals are induced with an optimal dose of 45 mg/kg body weight
of streptozotocin.
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| Page(s): | 867-874 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(12) [December 2012]
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