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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(12) [December 2012] >
| Title: | Curcumin sensitizes lung adenocarcinoma cells to apoptosis via intracellular redox status mediated pathway |
| Authors: | Kaushik, Gaurav
Kaushik, Toshi
Yadav, Subodh Kumar
Sharma, Sanjeev Kumar
Ranawat, Pavitra
Khanduja, Krishan Lal
Pathak, Chander Mohan |
| Keywords: | A549 Cells
Adenocarcinoma
Apoptosis
Curcumin
MAPK signaling
Reactive oxygen species
Redox status |
| Issue Date: | Dec-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The present study demonstrates that curcumin
acts as pro-oxidant and sensitizes human lung adenocarcinoma epithelial cells
(A549) to apoptosis via intracellular redox status mediated pathway. Results
indicated that curcumin induced cell toxicity (light microscopy and MTT assay)
and apoptosis (AnnexinV-FITC/PI labeling and caspase-3 activity) in these
cells. These events seem to be mediated through generation of reactive oxygen
species (ROS) and superoxide radicals (SOR) and enhanced levels of lipid
peroxidation. These changes were accompanied by increase in oxidized
glutathione (GSSG), reduced glutathione (GSH) and  -glutamylcysteine synthetase
(-GCS) activity, but decrease in GSH/GSSG ratio. The induction of apoptosis
and decrease in GSH/GSSG ratio was also accompanied by sustained
phosphorylation and activation of p38 mitogen activated protein kinase (MAPK).
On the other hand, addition of N-acetyl cysteine (NAC), an antioxidant, blocked
the curcumin-induced ROS production and rescued malignant cells from
curcumin-induced apoptosis through caspase-3 deactivation. However,
L-buthionine sulfoximine (BSO), a GSH synthesis blocking agent, further
enhanced curcumin-induced ROS production and apoptosis in A549 cells. Decreased
GSH/GSSG ratio seems to be a crucial factor for the activation of MAPK
signaling cascade by curcumin. The study therefore, provides an insight into
the molecular mechanism involved in sensitization of lung adenocarcinoma cells
to apoptosis by curcumin. |
| Page(s): | 853-861 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(12) [December 2012]
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