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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(12) [December 2012] >
| Title: | Effects of sodium dodecyl sulphate on enhancement of lipoxygenase activity of hemoglobin |
| Authors: | Ezebuo, F C
Eze, S O O
Chilaka, F C |
| Keywords: | Hemoglobin
Linoleic acid
Lipoxygenase
Met-hemoglobin
Oxodienes
Sodium dodecyl sulphate |
| Issue Date: | Dec-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Lipoxygenases comprise a family of non-heme iron-containing enzymes
that catalyze the stereospecific dioxygenation of polyunsaturated fatty acids
with 1, 4-cis-cis-pentadiene structure. Hemoglobin, a heme iron-containing
protein has been reported to have lipoxygenase activity but the assay
conditions that could enhance the activity remain obscure. Therefore,
establishment of optimum assay conditions for lipoxygenase activity of
hemoglobin could allow modeling of hemoglobin as lipoxygenase. Hemoglobin was extracted
from blood of an identified individual of genotype AA. The hemoglobin was
dialyzed at 4 oC for 24 h against 50 mM Tris-HCl buffers (pH 8.5 and 7.2) and effects of sodium
dodecyl sulphate (SDS) and linoleic studied at pH 5.0 and 7.2 with UV–VIS Titration Spectrophotometry. The results
show that 3.3, 8.6 and 88.1% concentrations of met-hemoglobin were found in
presence of 0.0 mM SDS at pH 5.0 and
7.2, 1.043 mM SDS at pH 7.2 and 0.404
mM SDS at pH 5.0 respectively. Also,
the difference spectra of hemoglobin in presence of linoleic acid showed
positive peak at 285 nm which suggest the presence of oxodienes–a reaction
product of hydroperoxidase activity of lipoxygenase. Formation of
met-hemoglobin/met-myoglobin is highly correlated with lipid oxidation. Since
highest concentration of met-hemoglobin (88.1%) was observed in presence of
0.404 mM SDS at pH 5.0, lipoxygenase
activity of hemoglobin was enhanced in presence of SDS under these conditions. |
| Page(s): | 847-852 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(12) [December 2012]
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