Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/15169
Title: <span style="font-size:11.0pt; mso-bidi-font-size:14.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN">Optimal<span style="font-size:11.0pt;mso-bidi-font-size: 14.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:HI" lang="EN"> <span style="font-size: 11.0pt;mso-bidi-font-size:14.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN">concentration of<span style="font-size:11.0pt; mso-bidi-font-size:14.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB"> hyaluronan<span style="font-size:11.0pt;mso-bidi-font-size:14.0pt; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN"> and<span style="font-size:11.0pt;mso-bidi-font-size:14.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Mangal; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB"> plant<span style="font-size:11.0pt;mso-bidi-font-size:14.0pt; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN"> protein<span style="font-size:11.0pt;mso-bidi-font-size:14.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Mangal; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB"> in different culture systems <span style="font-size:11.0pt; mso-bidi-font-size:14.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN">for<span style="font-size:11.0pt;mso-bidi-font-size: 14.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:HI" lang="EN"> <i style="mso-bidi-font-style: normal"><span style="font-size:11.0pt;mso-bidi-font-size:14.0pt; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN">in vitro</span></i><span style="font-size:11.0pt;mso-bidi-font-size:14.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Mangal; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN"> <span style="font-size:11.0pt;mso-bidi-font-size:14.0pt; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN">maturation<span style="font-size:11.0pt;mso-bidi-font-size:14.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Mangal; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN"> <span style="font-size:11.0pt;mso-bidi-font-size:14.0pt; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN">of bovine<span style="font-size:11.0pt;mso-bidi-font-size:14.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Mangal; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN"> <span style="font-size:11.0pt;mso-bidi-font-size:14.0pt; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN">oocytes </span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
Authors: Opiela, Jolanta
Latasiewicz, Ewa
Smorąg, Zdzisław
Keywords: Bovine oocytes
DNA fragmentation
Hyaluronan
IVM
TUNEL
Issue Date: Dec-2012
Publisher: NISCAIR-CSIR, India
Abstract: With a view to <span style="mso-bidi-font-family:" times="" new="" roman";="" mso-ansi-language:en"="" lang="EN">search for<span style="mso-ansi-language: EN" lang="EN"> <span style="mso-bidi-font-family:" times="" new="" roman";="" mso-ansi-language:en"="" lang="EN">optimal<span style="mso-ansi-language: EN" lang="EN"> <span style="mso-bidi-font-family:" times="" new="" roman";="" mso-ansi-language:en"="" lang="EN">concentration of<span style="mso-ansi-language:EN" lang="EN"> hyaluronan<span style="mso-ansi-language:EN" lang="EN"> (HA) <span style="mso-bidi-font-family:" times="" new="" roman";mso-ansi-language:en"="" lang="EN">and<span style="mso-ansi-language:EN" lang="EN"> plant<span style="mso-bidi-font-family:" times="" new="" roman";="" mso-ansi-language:en"="" lang="EN"> protein (PP)<span style="mso-ansi-language:EN" lang="EN"> in different culture systems <span style="mso-bidi-font-family:" times="" new="" roman";="" mso-ansi-language:en"="" lang="EN">for<span style="mso-ansi-language: EN" lang="EN"> <i style="mso-bidi-font-style:normal"><span style="mso-bidi-font-family:" times="" new="" roman";mso-ansi-language:en"="" lang="EN">in vitro</span></i><span style="mso-ansi-language:EN" lang="EN"> <span style="mso-bidi-font-family:" times="" new="" roman";="" mso-ansi-language:en"="" lang="EN">maturation<span style="mso-ansi-language: EN" lang="EN"> <span style="mso-bidi-font-family:" times="" new="" roman";="" mso-ansi-language:en"="" lang="EN">of bovine<span style="mso-ansi-language: EN" lang="EN"> <span style="mso-bidi-font-family:" times="" new="" roman";="" mso-ansi-language:en"="" lang="EN">oocytes, cumulus-oocyte complexes (COCs) were matured <i style="mso-bidi-font-style:normal">in vitro</i> in 2 culture systems (first co-cultured with granulose cells and estrus calf serum (ECS) in 2 mL volume, second without co-culture where ECS was replaced by exogenous hormones and BSA or PP in 100 µL dose under mineral oil). Seven types of media were used; 3 in first system and 4 in second system. <span style="mso-bidi-font-family:" times="" new="" roman";="" mso-ansi-language:en"="" lang="EN">To evaluate HA and PP effect on oocytes after <i style="mso-bidi-font-style:normal">in vitro</i> culture an estimation of meiosis stage and a level of DNA fragmentation was performed by TUNEL staining. The highest meiotic maturation (84%) was observed in oocytes cultured in medium enriched with ECS in co-culture with granulose cells (1<sup>st </sup>system). The lowest meiotic maturation was noted in medium with addition of BSA (43%). The addition of HA in the medium enriched with BSA significantly increased the rate of matured oocytes (67%) and also didn’t affect the chromatin quality of individual oocytes. The addition of HA to the culture medium supplemented with a PP decreased the rate of matured oocytes to 54% but no statistical differences were noted. The results of the present study showed that HA<span style="mso-bidi-font-family:" times="" new="" roman";mso-ansi-language:="" en-us"="" lang="EN-GB"> supplementation didn’t have a detrimental impact on oocyte chromatin integrity and improved bovine oocytes’ meiotic maturation in medium supplemented only with BSA without co-culture of granulose cells. </span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>
Description: 839-846
URI: http://hdl.handle.net/123456789/15169
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.50(12) [December 2012]

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