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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(12) [December 2012] >
| Title: | Optimal concentration of hyaluronan and plant protein in different culture systems for in vitro maturation of bovine oocytes |
| Authors: | Opiela, Jolanta
Latasiewicz, Ewa
Smorąg, Zdzisław |
| Keywords: | Bovine oocytes
DNA fragmentation
Hyaluronan
IVM
TUNEL |
| Issue Date: | Dec-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | With a view to search for optimal concentration of hyaluronan (HA) and plant protein (PP) in different culture systems for in
vitro maturation of bovine oocytes, cumulus-oocyte
complexes (COCs) were matured in vitro
in 2 culture systems (first co-cultured with granulose cells and estrus calf
serum (ECS) in 2 mL volume, second without co-culture where ECS was replaced by
exogenous hormones and BSA or PP in 100 µL dose under mineral oil). Seven types
of media were used; 3 in first system and 4 in second system. To evaluate HA and PP
effect on oocytes after in vitro
culture an estimation of meiosis stage and a level of DNA fragmentation was
performed by TUNEL staining. The highest meiotic maturation (84%) was observed
in oocytes cultured in medium enriched with ECS in co-culture with granulose
cells (1st system). The lowest meiotic maturation was noted in
medium with addition of BSA (43%). The addition of HA in the medium enriched
with BSA significantly increased the rate of matured oocytes (67%) and also
didn’t affect the chromatin quality of individual oocytes. The addition of HA
to the culture medium supplemented with a PP decreased the rate of matured
oocytes to 54% but no statistical differences were noted. The results of the
present study showed that HA supplementation didn’t have a
detrimental impact on oocyte chromatin integrity and improved bovine oocytes’
meiotic maturation in medium supplemented only with BSA without co-culture of
granulose cells.
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| Page(s): | 839-846 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(12) [December 2012]
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