20-Jun-2013
09:38:33 IST
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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Geo-Marine Sciences (IJMS) >
IJMS Vol.35 [2006] >
IJMS Vol.35(1) [March 2006] >
| Title: | Cryopreservation of the sperm of spotted halibut Verasper variegates (Pleuronectiformes, Pleuronectidae) |
| Authors: | Liu, Xuezhou
Zhang, Shicui
Zhang, Yongzhong
Xu, Yongjiang |
| Keywords: | Cryopreservation
Sperm
Spotted halibut
Verasper variegate |
| Issue Date: | Mar-2006 |
| Publisher: | CSIR |
| Abstract: | The sperm of spotted halibut Verasper variegates was cryopreserved by a simple and convenient protocol. The cryoprotectants used were dimethyl sulfoxide (Me₂SO), glycerol (Gly), methanol (MeOH), Me₂SO + egg yolk, Gly + egg yolk and MeOH + egg yolk. The percentage of forward motile sperm in semen cryopreserved in the different extenders with Me₂SO, Gly, MeOH, Me₂SO + egg yolk, Gly + egg yolk and MeOH + egg yolk was 40.5 ± 4.2%, 49.5 ± 4.2%, 38.3 ± 2.4%, 65.0 ± 4.0%, 67.5 ± 2.9% and 49.5 ± 8.4%, respectively. The fertilization and hatching rates of cryopreserved sperm were from 59 ± 2.4% to 70 ± 3.0%, and from 11 ± 3.0% to 18 ± 2.6%, respectively. These tended to be highest when Gly + egg yolk or Me₂SO + egg yolk were used as cryoprotectants. Under SEM, it could be seen that while the majority of the freeze-thawed sperm remained morphologically normal, some exhibited damaged or lost mitochondria, which possibly caused the decrease in motility and fertility of the freeze-thawed sperm |
| Page(s): | 24-28 |
| ISSN: | 0379-5136 |
| Source: | IJMS Vol.35(1) [March 2006]
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