NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Geo-Marine Sciences (IJMS) >
IJMS Vol.35 [2006] >
IJMS Vol.35(1) [March 2006] >

Title: Cryopreservation of the sperm of spotted halibut Verasper variegates (Pleuronectiformes, Pleuronectidae)
Authors: Liu, Xuezhou
Zhang, Shicui
Zhang, Yongzhong
Xu, Yongjiang
Keywords: Cryopreservation
Spotted halibut
Verasper variegate
Issue Date: Mar-2006
Publisher: CSIR
Abstract: The sperm of spotted halibut Verasper variegates was cryopreserved by a simple and convenient protocol. The cryoprotectants used were dimethyl sulfoxide (Me₂SO), glycerol (Gly), methanol (MeOH), Me₂SO + egg yolk, Gly + egg yolk and MeOH + egg yolk. The percentage of forward motile sperm in semen cryopreserved in the different extenders with Me₂SO, Gly, MeOH, Me₂SO + egg yolk, Gly + egg yolk and MeOH + egg yolk was 40.5 ± 4.2%, 49.5 ± 4.2%, 38.3 ± 2.4%, 65.0 ± 4.0%, 67.5 ± 2.9% and 49.5 ± 8.4%, respectively. The fertilization and hatching rates of cryopreserved sperm were from 59 ± 2.4% to 70 ± 3.0%, and from 11 ± 3.0% to 18 ± 2.6%, respectively. These tended to be highest when Gly + egg yolk or Me₂SO + egg yolk were used as cryoprotectants. Under SEM, it could be seen that while the majority of the freeze-thawed sperm remained morphologically normal, some exhibited damaged or lost mitochondria, which possibly caused the decrease in motility and fertility of the freeze-thawed sperm
Page(s): 24-28
ISSN: 0379-5136
Source: IJMS Vol.35(1) [March 2006]

Files in This Item:

File Description SizeFormat
IJMS 35(1) 24-28.pdf828.09 kBAdobe PDFView/Open
 Current Page Visits: 103 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 164215 since 01-Sep-2015  Last updated on 21-Jun-2016Webmaster: