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IJC-B Vol.51B(11) [November 2012] >


Title: Development of HPLC method for simultaneous estimation of ambroxol, guaifenesin and salbutamol in single dose form
Authors: Dubey, Nidhi
Sahu, Sandeep
Singh, G N
Keywords: Ambroxol
Guaifenesin
Salbutamol
HPLC method
Simultaneous determination
Issue Date: Nov-2012
Publisher: NISCAIR-CSIR, India
Abstract:   A simple, accurate and precise High Performance Liquid Chromatographic (HPLC) method has been developed for simultaneous determination of ambroxol, guaifenesin and salbutamol in single dosage form. The method has been validated as per the guidelines of ICH and FDA. The finalized Reverse Phase–HPLC method is revealed with significant shorter retention time of 15 min with simple isocratic program. The separation is achieved on C- 8, 5μm; 250 mm × 4.6 mm column with flow rate 1.0 mL per minute in isocratic mode using disodium hydrogen-ortho-phosphate buffer (pH 4.5) and methanol as mobile phase. Column oven temperature is maintained at 25°C and observations are recorded at 220 nm. The method is simple, accurate, reproducible and short and can be used for simultaneous analysis of ambroxol, guaifenesin and salbutamol in several single dose form formulation available in the market.
Page(s): 1633-1636
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0983(Online); 0376-4699(Print)
Source:IJC-B Vol.51B(11) [November 2012]

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