|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(11) [November 2012] >
| Title: | Cloning and expression of a small heat and salt tolerant protein (Hsp22) from Chaetomium globosum |
| Authors: | Aggarwal, Rashmi
Gupta, Sangeeta
Sharma, Sapna
Banerjee, Sagar
Singh, Priyanka |
| Keywords: | Chaetomium globosum
Gene cloning
Small heat shock protein
Stress tolerance |
| Issue Date: | Nov-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The present
study reports molecular characterization of small heat shock protein gene in
Indian isolates of
Chaetomium globosum, C. perlucidum, C. reflexum, C. cochlioides and C. cupreum. Six isolates of C. globosum and other species showed a
band of 630bp using specific primers. Amplified cDNA product of C. globosum (Cg 1) cloned and sequenced
showed 603bp open reading frame encoding 200 amino-acids. The protein sequence had a molecular mass of 22 kDa
and was therefore, named Hsp22. BlastX analysis revealed that the gene codes for a protein homologous to previously
characterized Hsp22.4 gene
from C. globosum (AAR36902.1,
XP 001229241.1) and shared 95%
identity in amino acid sequence. It also showed varying degree of similarities
with small Hsp protein from Neurospora spp. (60%), Myceliophthora sp. (59%), Glomerella
sp. (50%), Hypocrea sp. (52%), and Fusarium spp. (51%). This gene was
further cloned into pET28a (+) and transformed
E. coli BL21 cells were
induced by IPTG, and the expressed protein of 30 kDa was analyzed by SDS-PAGE.
The IPTG induced transformants
displayed significantly greater resistance to NaCl and Na2CO3
stresses.
|
| Page(s): | 826-832 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(11) [November 2012]
|
|
