Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14943
Title: Enhanced rosmarinic acid production in cultured plants of two species of <i style="mso-bidi-font-style:normal">Mentha</i>
Authors: Roy, Debleena
Mukhopadhyay, Sandip
Keywords: <i>Mentha</i>
Micropropagation
Phenylalanine
Rosmarinic acid
Tyrosine
Issue Date: Nov-2012
Publisher: NISCAIR-CSIR, India
Abstract:   In the present investigation an attempt has been made to enhance rosmarinic acid level in plants, grown<i style="mso-bidi-font-style: normal"> in vitro</i>, of 2 species of <i style="mso-bidi-font-style:normal">Mentha</i> in presence of 2 precursors in the nutrient media during culture. For <i style="mso-bidi-font-style: normal">in vitro</i> culture establishment and shoot bud multiplication, MS basal media were used supplemented with different concentrations and combinations of different growth regulator like NAA (α-napthaleneacetic acid), BAP (6-benzylaminopurine). The medium containing NAA (0.25 mg/L) and BAP (2.5 mg/L) gave the highest potentiality of shoot formation (average 58.0 numbers of shoots) per explant for <i style="mso-bidi-font-style:normal">Mentha piperita</i> L. and the medium containing BAP (2.0 mg/L) gave the highest potentiality of shoot (average 19.2 numbers of shoots) formation per explant for <i style="mso-bidi-font-style:normal">Mentha arvensis </i>L. The complete plants were regenerated in above mentioned media after 8 weeks of subculture. For <i style="mso-bidi-font-style:normal">in vitro</i> enhancement of rosmarinic acid production, the 2 precursors tyrosine (Tyr) and phenylalanine (Phe) were added in the nutrient media at different levels (0.5 mg/L to 15.0 mg/L). Tyrosine was found to be very effective for augmenting rosmarinic acid content in <i style="mso-bidi-font-style:normal">Mentha piperita</i> L. It nearly increased the production up to 1.77 times. In case of <i style="mso-bidi-font-style:normal">Mentha arvensis</i> L., phenylalanine significantly affected the production of rosmarinic acid and the production was nearly 2.03 times more than the control. No significant increase in biomass was observed after addition of these precursors indicating that the added amino acids acting as precursors for rosmarinic acid synthesis were readily utilized in producing rosmarinic acid without promoting growth. Total protein profile also revealed the presence of a specific band in polyacrylamide gel electrophoresis.
Description: 817-825
URI: http://hdl.handle.net/123456789/14943
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.50(11) [November 2012]

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