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Title: An efficient in vitro regeneration protocol for a natural dye yielding plant, Strobilanthes flaccidifolious Nees., from nodal explants
Authors: Deb, Chitta Ranjan
Arenmongla, T
Keywords: Micropropagation;Natural dye yielding plant;Nodal explants;Strobilanthes flaccidifolious;Tissue culture
Issue Date: Nov-2012
Publisher: NISCAIR-CSIR, India
Abstract:   Adventitious shoot buds formation from axillary buds of nodal segments of S. flaccidifolious was achieved on MS medium containing sucrose (3%, w/v), and α-naphthalene acetic acid (NAA; 3 µM) and benzyl adenine (3 µM) in combination. The nodal segments were primed on ‘Growtak Sieve’ for 48 h on MS medium containing sucrose (2%), polyvinyl pyrollidone (200 mgL-1) as antioxidant. About 80% of primed nodal segments responded positively and formed ~12 adventitious shoot buds per explants from explants collected during October-November months of every year. The shoot buds converted into plantlets on MS medium containing sucrose (3%) and kinetin (3 µM) where ~7 micro shoots developed per subculture after 8 weeks of culture. The regenerated micro shoots induced average 14 roots/ plant on medium containing NAA (3 µM). The regenerates were hardened for 6-7 weeks on medium with ½MS salt solution and sucrose (2%) under normal laboratory condition before transferring to potting mix. About 70% transplants survived after two months of transfer.
Page(s): 810-816
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.50(11) [November 2012]

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