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|Title:||In-vitro modulation of Na+ K+, Mg++ ATPases and AchE by sea anemone toxic proteins|
|Authors:||Ravindran, Vinoth S|
|Keywords:||Sea anemones;Proteins;Sodium pump;Acetylcholine;Neurotoxins;Enzyme|
|Abstract:||The crude as well as partially purified protein fractions of the sea anemone species viz. Heteractis magnifica, Stichodactyla haddoni and Paracodylactis sinensis, were collected from the Gulf of Mannar, southeast coast of India. These were found to modulate the functioning of the sodium pump (Na+ K+, Mg++ ATPase) at the presynaptic complex of neuro-transmission, thereby affecting ATP hydrolysis. They promote cholinestrase activity at the post synaptic complex by modulating the activity of the enzyme Acetyl Choline Esterase (AchE). Modulation was dose dependent. The proteins were toxic to mice. Histopathological observations reveal that the toxins affected the anatomy of the brain. Toxin injected mice showed various behavioral changes including manner shivering of fore limbs, loss of balance, opaque eyes, convulsions, paralysis, micturiction, flexing of muscles, prodding (insensitive to stimulii), dragging of hind limbs, rolling of tail, foaming from mouth and exophthalmia. This could be possibly correlated with CNS dysfunction.|
|ISSN:||0975-1033 (Online); 0379-5136 (Print)|
|Appears in Collections:||IJMS Vol.41(5) [October 2012]|
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