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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Geo-Marine Sciences (IJMS) >
IJMS Vol.41 [2012] >
IJMS Vol.41(5) [October 2012] >
| Title: | Isolation and characterization of fatty acid esters and phosphatidylethanolamine surfactants from a consortium of marine bacteria |
| Authors: | Deshmukh, Chitra
Jagtap, C.B.
Titus, Susan
Kumar, Pradeep |
| Keywords: | Biosurfactant
High speed diesel (HSD)
Surface tension
Phospholipid
Bioremediation
Oil pollution |
| Issue Date: | Oct-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Â Consortium of five bacterial isolates from
marine environment produced extracellular surface-active metabolites during
growth in culture medium containing High Speed Diesel (HSD) as the sole source
of carbon and energy. Product obtained was characterized for its surface-active
properties and chemical constituents. It was found to reduce the surface
tension of distilled water from 72 to 36 mN/m. Fourier Transform Infra Red
(FTIR) spectrum revealed the presence of functional groups such as alkyl,
ester, carboxyl, amine and alcohol indicating its structural similarity to
mixture of fatty acid esters and amino group containing lipids. Major
constituent was lipids (74.49%) which on further analysis by Thin Layer
Chromatography (TLC) and Gas Chromatography – Mass Spectroscopy (GCMS) showed
the presence of amino group containing phospholipids and different fatty acids.
Thermogravimetric analysis indicated the material to be thermostable (Initial
Decomposition Temperature: IDT = 198oC). Characterization therefore
indicated the reported biosurfactant to be a mixture of hydrophobic and
hydrophilic moieties facilitating the amphiphilic behaviour useful for the
effective emulsification and dispersion of petroleum hydrocarbons. |
| Page(s): | 398-404 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1033 (Online); 0379-5136 (Print) |
| Source: | IJMS Vol.41(5) [October 2012]
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