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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.45 [2008] >
IJBB Vol.45(2) [April 2008] >
| Title: | Identification and partial characterization of juvenile hormone esterase from cotton pest Dysdercus cingulatus |
| Authors: | Elayidam, U Gayathri
Muraleedharen, D |
| Keywords: | Dysdercus cingulatus
Juvenile Hormone Esterase
Isoforms
PCR
cDNA |
| Issue Date: | Apr-2008 |
| Publisher: | CSIR |
| Abstract: | Juvenile hormone esterase (JHE), a selective enzyme that
hydrolyzes the methyl ester of insect juvenile hormone plays an
important role in regulating metamorphosis in nymphs as well as
reproduction in adults. Studies on JH degradation provide insight
into the possibilities of physiological disruption in the insects. In
the present study, the JH degrading enzyme, JHE from the cotton
pest Dysdercus cingulatus (Heteroptera) is characterized.
Electrophoretic analysis of haemolymph during various
developmental stages showed the JHE bands prominent only on
the final day of 5th instar nymph, and the esterase substrate
specificity confirmed the presence of JHE isoforms. In an attempt
to clone cDNA of JHE gene from the final instar nymphs, mRNA
isolated from fat bodies was coupled with JHE gene-specific
primers and the cDNA was synthesized using RT-PCR. The PCR
amplified cDNA showed the presence of JHE isoforms in
D. cingulatus. |
| Page(s): | 121-125 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.45(2) [April 2008]
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