NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(5) [October 2012] >


Title: Suppression of apoptosis leads to cisplatin resistance in V79 cells subjected to chronic oxidative stress
Authors: Ghosh, Rita
Girigoswami, Koyeli
Guha, Dipanjan
Keywords: Chronic oxidative stress
Cisplatin resistance
Apoptosis
Cytochrome c
Caspase 9
Caspase 3
Issue Date: Oct-2012
Publisher: NISCAIR-CSIR, India
Abstract: We derived V79C cells from V79 cell line through chronic oxidative stress by H2O2. These cells demonstrated transformation-like stable changes. Our objective was to see how V79C cells would respond to cisplatin treatment and also to understand the mechanism of cisplatin-resistance, because resistance towards various chemotherapeutic agents is major cause of concern in cancer therapeutics. The sensitivity to cisplatin in these cells was observed by comparing the viability with that of parental V79 cells from colonogenic assay. The role of apoptotic death was investigated microscopically by Hoechst staining and from nucleosomal ladder formation in agarose gel. Release of cytochrome c from the mitochondria was determined by Western blotting. Caspase 9 and caspase 3 activities were estimated through fluorimetric assay. We found that V79C cells exhibited lower sensitivity towards killing by cisplatin through suppression of apoptotic cell death. Quantifying the release of cytochrome c in the cytoplasm and assay of caspase 9 and caspase 3 activities revealed that cisplatin resistance was due to inhibition of caspase-dependent apoptotic death pathways. These findings may aid in understanding the mechanism of cisplatin resistance in tumors arising from oxidative stress. Exogenous caspases may facilitate apoptotic death to sensitize such resistant cells.
Page(s): 363-370
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Source:IJBB Vol.49(5) [October 2012]

Files in This Item:

File Description SizeFormat
IJBB 49(5) 363-370.pdf664.51 kBAdobe PDFView/Open
 Current Page Visits: 660 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 607018 since 06-Feb-2009  Last updated on 31-Oct-2014Webmaster: nopr@niscair.res.in