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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(5) [October 2012] >
| Title: | Suppression of apoptosis leads to cisplatin resistance in V79 cells subjected to chronic oxidative stress |
| Authors: | Ghosh, Rita
Girigoswami, Koyeli
Guha, Dipanjan |
| Keywords: | Chronic oxidative stress
Cisplatin resistance
Apoptosis
Cytochrome c
Caspase 9
Caspase 3 |
| Issue Date: | Oct-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | We derived V79C cells from V79
cell line through chronic oxidative stress by H2O2. These
cells demonstrated transformation-like stable changes. Our objective was to see
how V79C cells would respond to cisplatin treatment and also to
understand the mechanism of cisplatin-resistance, because resistance towards
various chemotherapeutic agents is major cause of concern in cancer
therapeutics. The sensitivity to cisplatin in these cells was observed by comparing
the viability with that of parental V79 cells from colonogenic assay. The role
of apoptotic death was investigated microscopically by Hoechst staining and
from nucleosomal ladder formation in agarose gel. Release of cytochrome c from the mitochondria was determined
by Western blotting. Caspase 9 and caspase 3 activities were estimated through
fluorimetric assay.
We found that V79C cells exhibited lower sensitivity towards killing
by cisplatin through suppression of apoptotic cell death. Quantifying the release
of cytochrome c in the cytoplasm and
assay of caspase 9 and caspase 3 activities revealed that cisplatin resistance
was due to inhibition of caspase-dependent apoptotic death pathways. These
findings may aid in understanding the mechanism of cisplatin resistance in
tumors arising from oxidative stress. Exogenous caspases may facilitate
apoptotic death to sensitize such resistant cells. |
| Page(s): | 363-370 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(5) [October 2012]
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