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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(5) [October 2012] >
| Title: | Curcumin delays endometriosis development by inhibiting MMP-2 activity |
| Authors: | Jana, Sayantan
Rudra, Deep Sankar
Paul, Sumit
Swarnakar, Snehasikta |
| Keywords: | Curcumin
Endometriosis
Matrix metalloproteinase
Tissue inhibitor of MMPs |
| Issue Date: | Oct-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Endometriosis is a common reproductive
disorder believed to be associated with matrix metalloproteinases (MMPs)
activities for invasion and remodeling of endometrial tissues. Ectopic
endometrium has higher capacity to produce proMMP-2 than eutopic tissues;
however, the role of MMP-2 during early phase of endometriosis development is
still unclear. In the present study, we investigated the role of MMP-2 in
establishment and development of endometriosis in mouse model. The effect of
curcumin on regression of endometriosis through protease/antiprotease balance
between MMP-2 and TIMP-2 was also examined. After endometrial inoculation into
peritoneum, we observed a significant elevation of proMMP-2 activity from day 2
onwards. This increased MMP-2 activity was associated with decreased expression
of tissue inhibitor of MMP (TIMP)-2, while a significant up-regulation of
active MMP-2 activity was observed from day 3 onwards. The activation of
proMMP-2 to active MMP-2 was associated with increased expression of membrane type 1
matrix metalloproteinase (MT1MMP). Curcumin at a dose
of 48 mg/kg b.w. repressed the MMP-2 activity via up-regulation of bound TIMP-2
expression, thus delayed endometriosis development. In addition, curcumin
inhibited production of active MMP-2 by down-regulating MT1MMP expression.
Moreover, endometriotic progression was directly linked with increased
MMP-2/TIMP-2 ratio which was delayed by curcumin pretreatment. In summary, our
study documents the regulation of MMP-2 activity by TIMP-2 during the early
phase of endometriosis development and inhibitory action of curcumin thereon.
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| Page(s): | 342-348 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(5) [October 2012]
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