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Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
IJNPR Vol.3 [2012] >
IJNPR Vol.3(3) [September 2012] >
| Title: | Substrate suitability of neem seed kernel for the growth and elaboration of aflatoxins by Aspergillus parasiticus (NRRL 2999) |
| Authors: | Tanuja, Kosuri
Kavitha, Godugu
Karuna, Rupula
Sashidhar, R B |
| Keywords: | Aflatoxin
Aspergillus parasiticus
Azadirachtin
Neem seed kernel
Polyphenols
Proximate composition |
| Issue Date: | Sep-2012 |
| Publisher: | NISCAIR-CSIR, India |
| IPC Code: | Int. cl. (2011.01) - A61K 36/58, A61K 131/00, A61P 31/10 |
| Abstract: | Neem seed kernels
artificially infested with Aspergillus
parasiticus (NRRL 2999) was evaluated for aflatoxin elaboration and fungal
growth, and compared with groundnut, a high risk commodity for aflatoxin
contamination. At optimal moisture content (10-12%) the total, individual
toxins (AFB1, AFB2, AFG1 and AFG2)
and ergosterol content increased and showed maximum levels on day 9. Crude
protein and polyphenols increased while, fat and total sugar content decreased
during the period of infection. The protein content correlated positively (r = 0.734) with total toxin levels,
whereas fat content
(r = -0.761) and total sugars (r = -0.891) showed negative correlation and
they were all statistically significant (p<0.01).
The polyphenols showed negative and non-significant correlation with total
toxin levels. Azadirachtin one of the major active principles of neem seed
kernel showed significant decrease on day 3 (P<0.05) and day 6 (P<0.01).
Neem seed kernel has shown 54 and 74% less aflatoxin production on day 9 and
12, respectively in comparison to groundnut seeds. Ergosterol content also
showed 60% decrease on day 9, conferring it a poor substrate for fungal growth
and aflatoxin elaboration. |
| Page(s): | 395-406 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0976-0512 (Online); 0976-0504 (Print) |
| Source: | IJNPR Vol.3(3) [September 2012]
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