NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.45 [2008] >
IJBB Vol.45(2) [April 2008] >


Title: Optimized culture conditions for bacteriocin production by Pediococcus acidilactici LAB 5 and its characterization
Authors: Mandal, Vivekananda
Sen, Sukanta Kumar
Mandal, Narayan Chandra
Keywords: Antibacterial
Bacteriocin
Cystibiotics
Pediococcus acidilactici
Issue Date: Apr-2008
Publisher: CSIR
Abstract: A strain of Pediococcus acidilactici LAB 5 was isolated from vacuum-packed fermented meat product, in order to obtain a novel bacteriocin from food-grade organisms. Optimized culture conditions for bacteriocin production in different media (viz., MRS, TGE, TGE + buffer, TGE + Tween 80, and TGE + Tween 80 + buffer) and at different temperatures and pH conditions were reported. TGE + Tween 80 + buffer medium was found to be most effective for bacteriocin production (about 2,400 AU/ ml) by this strain, when incubated at 37°C for 24 h. Bacteriocin, partially purified by adsorptiondesorption method showed molecular mass of 10.3 kDa and produced prominent inhibition zone in activity gel. It showed significant storage stability both at high as well as in low temperatures for up to 6 months and retained its activity in a number of organic solvents, except in 2-mercaptoethanol. The treatment with amylase and lysozyme did not change its activity, but it lost its activity on proteinase K treatment. Antibacterial efficacy of bacteriocin was proved against some food spoilage and human pathogenic bacteria like Enterococcus, Leuconostoc, Listeria, Staphylococcus and Streptococcus.
Page(s): 106-110
ISSN: 0301-1208
Source:IJBB Vol.45(2) [April 2008]

Files in This Item:

File Description SizeFormat
IJBB 45(2) 106-110.pdf346.16 kBAdobe PDFView/Open
 Current Page Visits: 843 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 592721 since 06-Feb-2009  Last updated on 18-Sep-2014Webmaster: nopr@niscair.res.in