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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(10) [October 2012] >
| Title: | Protocol for augmented shoot organogenesis in selected variety of soybean [Glycine max L. (Merr.)] |
| Authors: | Devi, M K Akitha
Sakthivelu, G
Giridhar, P
Ravishankar, G A |
| Keywords: | Cotyledonary node
Glycine max
In vitro rooting
Microshoots
Shoot buds
Triacontanol |
| Issue Date: | Oct-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Development of a reproducible, versatile and efficient in vitro plant regeneration system is
highly warranted for Indian soybean varieties for their mass multiplication in
view of their commercial significance. Accordingly a protocol for direct shoot
organogenesis in soybean variety JS 335 has been developed. Using cotyledonary
node explants significant organogenic responses, mean shoot number and shoot
length were observed when these were incubated on MS medium supplemented with 0.89
µM Benzyladenine (BA) and
5 μg/L triacontanol (TRIA) where in 9.3 ± 0.5 shoots
were obtained. TRIA at 5 μg /L able to
produce 6.8 ± 0.5 shoot buds in presence of 0.98 µM IBA and 0.89 µM BA.
Highest mean shoot buds (14.0 ± 0.5 and 9.0 ± 0.5) and mean shoot length (4.6 ±
0.3 and 10.0 ± 0.7) were obtained when cotyledonary node and shoot tip explants
were cultured on MS medium containing 0.14 µM
gibberellic acid (GA3), 0.89 µM
BA and 5 μg/L TRIA. Moreover, TRIA supported highest mean
root number (6.3±0.5) and root length (21.5 ± 0.57 cm). Field survival of in vitro derived plants of TRIA
treatment was 70% and the overall growth and seed yield was also significantly
better than control plants. This protocol may be used for improving the in vitro regeneration of soybean variety
JS 335 for transformation studies.
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| Page(s): | 729-734 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(10) [October 2012]
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