Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14797
Title: Protocol for augmented shoot organogenesis in selected variety of soybean [<i>Glycine</i><i style="mso-bidi-font-style: normal"> max</i> L. (Merr.)]
Authors: Devi, M K Akitha
Sakthivelu, G
Giridhar, P
Ravishankar, G A
Keywords: Cotyledonary node
<i><span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA; mso-bidi-font-weight:bold" lang="EN-GB">Glycine max</span></i>
<i><span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA; mso-bidi-font-weight:bold" lang="EN-GB">In vitro</span></i><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language: EN-US;mso-bidi-language:AR-SA;mso-bidi-font-weight:bold" lang="EN-GB"> rooting</span>
Microshoots
Shoot buds
Triacontanol
Issue Date: Oct-2012
Publisher: NISCAIR-CSIR, India
Abstract: <span style="mso-bidi-font-size:9.0pt;letter-spacing: -.1pt" lang="EN-GB">Development of a reproducible, versatile and efficient <i style="mso-bidi-font-style:normal">in vitro</i> plant regeneration system is highly warranted for Indian soybean varieties for their mass multiplication in view of their commercial significance. Accordingly a protocol for direct shoot organogenesis in soybean variety JS 335 has been developed. Using cotyledonary node explants significant organogenic responses, mean shoot number and shoot length were observed when these were incubated on MS medium supplemented with 0.89 µ<i style="mso-bidi-font-style:normal">M</i> Benzyladenine (BA) and 5 <span style="mso-bidi-font-size:9.0pt;mso-fareast-font-family: TimesNewRoman;letter-spacing:-.1pt" lang="EN-GB">μ<span style="mso-bidi-font-size: 9.0pt;letter-spacing:-.1pt" lang="EN-GB">g/L triacontanol (TRIA) where in 9.3 ± 0.5 shoots were obtained. TRIA at 5 <span style="mso-bidi-font-size: 9.0pt;mso-fareast-font-family:TimesNewRoman;letter-spacing:-.1pt" lang="EN-GB">μ<span style="mso-bidi-font-size:9.0pt;letter-spacing:-.1pt" lang="EN-GB">g /L able to produce 6.8 ± 0.5 shoot buds in presence of 0.98 µ<i style="mso-bidi-font-style: normal">M</i> IBA and 0.89 µ<i style="mso-bidi-font-style:normal">M</i> BA. Highest mean shoot buds (14.0 ± 0.5 and 9.0 ± 0.5) and mean shoot length (4.6 ± 0.3 and 10.0 ± 0.7) were obtained when cotyledonary node and shoot tip explants were cultured on MS medium containing 0.14 µ<i style="mso-bidi-font-style:normal">M</i> gibberellic acid (GA<sub>3</sub>), 0.89 µ<i style="mso-bidi-font-style:normal">M</i> BA and 5<span style="mso-bidi-font-size:9.0pt;mso-fareast-font-family: TimesNewRoman;letter-spacing:-.1pt" lang="EN-GB"> μ<span style="mso-bidi-font-size: 9.0pt;letter-spacing:-.1pt" lang="EN-GB">g/L TRIA. Moreover, TRIA supported highest mean root number (6.3±0.5) and root length (21.5 ± 0.57 cm). Field survival of <i style="mso-bidi-font-style:normal">in vitro</i> derived plants of TRIA treatment was 70% and the overall growth and seed yield was also significantly better than control plants. This protocol may be used for improving the <i style="mso-bidi-font-style:normal">in vitro</i> regeneration of soybean variety JS 335 for transformation studies. </span></span></span></span></span></span></span>
Description: 729-734
URI: http://hdl.handle.net/123456789/14797
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.50(10) [October 2012]

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