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JSIR Vol.71 [2012] >
JSIR Vol.71(09) [September 2012] >
| Title: | Sequential cellulase production, saccharification and ethanol fermentation using rice straw |
| Authors: | Pasha, Chand
Sekhar, Banoth Chandra
Srinivas, Banoth
Balakrishna, Kannoju
Hanumalal, Nunavath |
| Keywords: | A. niger
Cellulases
Rice straw
Sequential saccharification and fermentation
S. cerevisiae (OC21) |
| Issue Date: | Sep-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This study presents alkali pretreated rice straw for
cellulase production using Aspergillus niger CP1 by solid state
fermentation (SSF). On 8th day, 140±2.4 IU g/l dry substrate (ds) carboxy
methyl cellulose CMCase), 15±1.2 IU g/l ds filter paper activity (FPA) and
24±1.1 IU g/l ds â-glucosidase activities were noted. On extraction of 120 g
material with 1 l of 0.1 mol/l citrate buffer, 5.9±0.12 IU ml/l CMCase,
0.62±0.001 IU ml/l FPA and 1.11±0.002 IU/ml â-glucosidase were obtained. When
extracted enzyme was used for saccharification of 120 g fresh pretreated rice
straw containing 100 g holocellulose, 45±0.2 g, sugars (50 g/l) were released
in 900 ml hydrolysate to give 45% hydrolysis. Hydrolysate after yeast
fermentation gave 15.6±0.05 g/l ethanol with a yield of 0.4±0.011 g/l/h and 78%
fermentation efficiency. In sequential enzyme production, saccharification and
fermentation studies, on 8thday of cellulase production by SSF, temperature was
shifted from 28±1°C to 55°C for 36 h, and 76% hydrolysis
was achieved. When A. niger in hydrolysate was
inactivated and used, 68±0.83 g/l ethanol and 92% fermentation efficiency were
obtained. Hydrolysate used without inactivation of A. niger gave 61.65±0.78 g/l
ethanol and 85% fermentation efficiency. |
| Page(s): | 616-620 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.71(09) [September 2012]
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