Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14632
Title: Sequential cellulase production, saccharification and ethanol fermentation using rice straw
Authors: Pasha, Chand
Sekhar, Banoth Chandra
Srinivas, Banoth
Balakrishna, Kannoju
Hanumalal, Nunavath
Keywords: <i><span style="font-size:9.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-IN;mso-fareast-language: EN-IN;mso-bidi-language:AR-SA">A. niger</span></i>
Cellulases
Rice straw
Sequential saccharification and fermentation
<i><span style="font-size:9.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-IN;mso-fareast-language: EN-IN;mso-bidi-language:AR-SA">S. cerevisiae </span></i><span style="font-size: 9.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language:AR-SA">(<i>OC21)</i></span>
Issue Date: Sep-2012
Publisher: NISCAIR-CSIR, India
Abstract: This study presents alkali pretreated rice straw for cellulase production using <i>Aspergillus niger </i>CP1 by solid state fermentation (SSF). On 8th day, 140±2.4 IU g/l dry substrate (ds) carboxy methyl cellulose CMCase), 15±1.2 IU g/l ds filter paper activity (FPA) and 24±1.1 IU g/l ds â-glucosidase activities were noted. On extraction of 120 g material with 1 l of 0.1 mol/l citrate buffer, 5.9±0.12 IU ml/l CMCase, 0.62±0.001 IU ml/l FPA and 1.11±0.002 IU/ml â-glucosidase were obtained. When extracted enzyme was used for saccharification of 120 g fresh pretreated rice straw containing 100 g holocellulose, 45±0.2 g, sugars (50 g/l) were released in 900 ml hydrolysate to give 45% hydrolysis. Hydrolysate after yeast fermentation gave 15.6±0.05 g/l ethanol with a yield of 0.4±0.011 g/l/h and 78% fermentation efficiency. In sequential enzyme production, saccharification and fermentation studies, on 8thday of cellulase production by SSF, temperature was shifted from 28±1°C to 55°C for 36 h, and 76% hydrolysis was achieved. When <i>A. niger </i>in hydrolysate was inactivated and used, 68±0.83 g/l ethanol and 92% fermentation efficiency were obtained. Hydrolysate used without inactivation of <i>A. niger </i>gave 61.65±0.78 g/l ethanol and 85% fermentation efficiency.
Description: 616-620
URI: http://hdl.handle.net/123456789/14632
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.71(09) [September 2012]

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