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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(09) [September 2012] >
| Title: | Effect of methanolic and water extract of Leucobryum bowringii Mitt. on growth, migration and invasion of MCF 7 human breast cancer cells in vitro |
| Authors: | Manoj, G S
Kumar, T R Santhosh
Varghese, Saneesh
Murugan, K |
| Keywords: | Adhesion
Breast cancer cells
Invasion
Leucobryum bowringii
Metalloproteinase
Migration |
| Issue Date: | Sep-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Inhibitory effects of methanol and water extract of L. bowringii. on the
adhesion, migration, invasion and matrix metalloproteinase (MMP) activities of
MCF 7 human breast cancer cell line are reported. Cells were cultured with 10,
25,
50 µg/mL methanolic or water extract of L. bowringii. Culture medium
containing 0.1% DMSO was used as a solvent control. Ultra structural analysis
by electron microscopy revealed typical features of apoptosis. A remarkable
dose-response parallelism was observed between methanolic extract with growth,
migration and invasion of breast cancer cells. Fractionation of methanolic
extract by RP-HPLC revealed a pool of phenolic acids. Hoechst 33342 staining
assay reveals massive chromatin condensation and subsequent cleavage of
structural components of nucleus. The results indicate that methanol extracts
inhibit the growth of human breast cancer cells partially through the
inhibition of metallo proteinases MMP-2 and MMP-9 activities. Methanolic
extract has more anti-metastatic effects in cell based assay than water extract.
Clinical application of L. bowringii extract as a bioactive
chemopreventive compound may be helpful in limiting breast carcinoma invasion
and metastasis. |
| Page(s): | 602-611 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(09) [September 2012]
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