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|Title:||<span style="font-size:13.0pt;mso-bidi-font-size: 10.0pt" lang="EN-GB">Indirect organogenesis from nodal explants of <i>Coccinia grandis</i><span style="mso-bidi-font-style:italic"> (L.) Voigt </span></span>|
Rao, M V
|Abstract:||<span style="mso-bidi-font-size:9.0pt" lang="EN-GB">The present study reports an efficient protocol for indirect shoot organogenesis and plantlets regeneration of <i>Coccinia grandis</i> (L.) Voigt from nodal explants. The maximum frequency of organogenic callus induction was observed in MS medium supplemented with 0.1 mg/L 1-naphthaleneacetic acid (NAA) and 1.0 mg/L N<sup>6</sup>-benzyladenine (BA). Multiple shoot induction was achieved from the surface of callus on regeneration medium [MS nutrients supplemented with NAA, BA and various concentrations of kinetin (KIN) 0.1-2.0 mg/L]. The highest shoot multiplication (90%) with the increased numbers of shoots (8.7±1.8) and the shoot mean length (8.8±0.4 cm) was achieved on MS medium with 0.1 mg/L NAA, 1.0 mg/L BA and 0.5 mg/L KIN. The regenerated shoots were rooted <i>in vitro</i> on MS medium containing 0.1 mg/L IBA. Plantlets with well developed shoot and root systems were successfully acclimatized (83.5%) and exhibited normal morphology and growth characteristics. </span>|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.11(3) [July 2012]|
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