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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | Indirect organogenesis from nodal explants of Coccinia grandis (L.) Voigt |
| Authors: | Thiripurasundari, U
Rao, M V |
| Keywords: | Coccinia grandis
Indirect organogenesis
Nodal explants |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The present
study reports an efficient protocol for indirect shoot organogenesis and
plantlets regeneration of Coccinia grandis (L.) Voigt from nodal
explants. The maximum frequency of organogenic callus induction was observed in
MS medium supplemented with 0.1 mg/L 1-naphthaleneacetic acid (NAA)
and 1.0 mg/L N6-benzyladenine (BA). Multiple shoot induction was
achieved from the surface of callus on regeneration medium [MS nutrients
supplemented with NAA, BA and various concentrations of kinetin (KIN) 0.1-2.0
mg/L]. The highest shoot multiplication (90%) with the increased numbers of
shoots (8.7±1.8) and the shoot mean length (8.8±0.4 cm) was achieved on MS
medium with 0.1 mg/L NAA, 1.0 mg/L BA and 0.5 mg/L KIN. The regenerated shoots
were rooted in vitro on MS medium containing 0.1 mg/L IBA. Plantlets
with well developed shoot and root systems were successfully acclimatized
(83.5%) and exhibited normal morphology and growth characteristics.
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| Page(s): | 352-354 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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