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dc.contributor.authorSaxena, Sonal-
dc.contributor.authorSharma, Amit-
dc.contributor.authorSardana, Jyoti-
dc.contributor.authorSharma, Madan Mohan-
dc.contributor.authorBatra, Amla-
dc.identifier.issn0975-0967 (Online); 0972-5849 (Print)-
dc.description.abstractAn efficient protocol has been developed for high frequency plant regeneration through somatic embryogenesis in the biodiesel plant, Jatropha curcus. Cotyledonary leaves were used for the development of the embryogenic callus on MS medium with 0.2 mg/L indole-3-acetic acid (IAA), which developed within 3-4 wk. Later, somatic embryos developed on MS medium with IAA (0.2 mg/L) along with 6-benzyl aminopurine (BAP; 1.5 mg/L). Fist, globular embryo clumps were seen within 3-4 wk and then they got transformed into heart, torpedo and cotyledonary shaped embryos on the same medium after 6 wk. On further subculture on the same medium, embryos germinated with shoot and root primordia, which ultimately gave rise to complete plantlets within 4-5 wk. The pantlets were hardened and transferred to the soil. Around 50% plantlets survived in the soil.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBT Vol.11(3) [July 2012]en_US
dc.subjectCotyledonary shapeen_US
dc.subjectEmbryogenic callusen_US
dc.subjectHeart shapeen_US
dc.subjectJatropha curcusen_US
dc.subjectTorpedo shapeen_US
dc.titleSomatic embryogenesis in Jatropha curcus L. using cotyledonary leavesen_US
Appears in Collections:IJBT Vol.11(3) [July 2012]

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